Human Reproduction, Vol 13, 3449-3455, Copyright © 1998 by Oxford University Press
L Hewitson, D Takahashi, T Dominko, C Simerly and G Schatten
Notwithstanding the thousands of seemingly healthy children born after
intracytoplasmic sperm injection (ICSI), it is not yet possible to conclude
absolutely that the ICSI procedure might induce some altered development or
that the ICSI protocol might not be improved even further. To address this
in a clinically relevant system, the developmental potential of rhesus
monkey embryos produced by ICSI is reported. Oocytes collected by
laparoscopy from gonadotrophin- stimulated fertile females were fertilized
by ICSI using spermatozoa obtained from fertile males by
electro-ejaculation. Neither sperm immobilization prior to injection nor an
additional chemical stimulus were necessary to achieve oocyte activation
and pronuclear formation. Survival and activation of the injected oocytes
were judged by the extrusion of the second polar body. Successful
fertilization was confirmed by the presence of two pronuclei within 12 h
post-ICSI. Some oocytes were fixed and processed for the detection of
microtubules and chromatin. Fluorescent labelling revealed that by 12 h
post-ICSI the male and female pronuclei were closely apposed and
eccentrically positioned within a large microtubule aster. ICSI resulted in
a 76.6 +/- 14.9% fertilization rate. First cleavage was completed within 24
h post- ICSI. Two-cell ICSI embryos were co-cultured in CMRL medium on a
buffalo rat liver cell monolayer until the hatched blastocyst stage.
Oocytes collected laparoscopically from stimulated monkeys can be
fertilized by ICSI and will complete preimplantation embryo development in
vitro demonstrating that the rhesus monkey is an excellent preclinical
model for examining and understanding many aspects of human ICSI.
ARTICLES
Fertilization and embryo development to blastocysts after intracytoplasmic sperm injection in the rhesus monkey
Division of Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton 97006, USA.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M.-W. Li, S. Meyers, T. L. Tollner, and J. W. Overstreet Damage to Chromosomes and DNA of Rhesus Monkey Sperm Following Cryopreservation J Androl, July 1, 2007; 28(4): 493 - 501. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Hewitson Primate models for assisted reproductive technologies Reproduction, September 1, 2004; 128(3): 293 - 299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ramalho-Santos, P. Sutovsky, C. Simerly, R. Oko, G. M. Wessel, L. Hewitson, and G. Schatten ICSI choreography: fate of sperm structures after monospermic rhesus ICSI and first cell cycle implications Hum. Reprod., December 1, 2000; 15(12): 2610 - 2620. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Gabriel Sánchez-Partida, G. Maginnis, T. Dominko, C. Martinovich, B. McVay, J. Fanton, and G. Schatten Live Rhesus Offspring by Artificial Insemination Using Fresh Sperm and Cryopreserved Sperm Biol Reprod, October 1, 2000; 63(4): 1092 - 1097. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. W.S. Chan, C.M. Luetjens, T. Dominko, J. Ramalho-Santos, C. R. Simerly, L. Hewitson, and G. Schatten Foreign DNA transmission by ICSI: injection of spermatozoa bound with exogenous DNA results in embryonic GFP expression and live Rhesus monkey births Mol. Hum. Reprod., January 1, 2000; 6(1): 26 - 33. [Abstract] [Full Text] [PDF]
|
|