Human Reproduction, Vol 13, 3478-3486, Copyright © 1998 by Oxford University Press
PG Lalitkumar, J Sengupta, AA Karande and D Ghosh
Placental protein 14 (PP14) is a glycoprotein which is secreted by
secretory phase endometrium and decidua in women. Despite the suggestion
that PP14 is involved in the process of endometrial maturation for
blastocyst implantation, our understanding in this regard is poor. In the
present study, the concentrations and distribution patterns of
immunodetectable PP14 in the endometrium during proliferative and secretory
phases of normal ovulatory menstrual cycles, as well as in implantation
stage endometrium in naturally mated ovulatory cycles with or without early
luteal phase mifepristone treatment, were investigated using the rhesus
monkey as a primate model. Immunopositive PP14 was observed mainly in
epithelial cells of glands and it was detected in one major immunopositive
band at Mr 28 kDa in tissue homogenate and spent medium. The area of
immunopositive precipitation of PP14 in glands was minimal in follicular
phase endometrium, and was higher (P < 0.01) in early, mid- and late
luteal phase endometrium compared with that in pre- and periovulatory
phases of the cycle, but there was no change in its area profile in the
glandular compartment throughout the luteal phase. Immunopositivity for
PP14 in luminal contents of gland displayed an increasing profile from
early to late secretory phases. Thus, the concentrations and the
distribution of immunodetectable PP14 in luteal phase endometrium of the
rhesus monkey showed marked similarity with those of human endometrium
during the natural menstrual cycle. Although there was no marked change in
the band characterstics for the protein in implantation stage endometrium
following early luteal phase mifepristone treatment, it was markedly
decreased (P < 0.01) in tissue homogenate and in vitro spent medium
along with a lesser (P < 0.02) degree of immunoprecipitation in the
glands in implantation stage samples of mifepristone treatment group
compared with that in control group samples. Thus, the contragestional
effect of early luteal phase mifepristone treatment appears to be
associated with a decrease in the concentration of immunodetectable PP14 in
implantation stage endometrial glands and its secretion in the rhesus
monkey. It remains to be seen whether this decline is caused from direct
antiprogesterone action on endometrial glands during progesterone
dominance, or secondarily from associated retarded development of
endometrium.
ARTICLES
Placental protein 14 in endometrium during menstrual cycle and effect of early luteal phase mifepristone administration on its expression in implantation stage endometrium in the rhesus monkey
Department of Physiology, All India Institute of Medical Sciences, New Delhi.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  P.G.L. Lalitkumar, S. Lalitkumar, C.X. Meng, A. Stavreus-Evers, F. Hambiliki, U. Bentin-Ley, and K. Gemzell-Danielsson Mifepristone, but not levonorgestrel, inhibits human blastocyst attachment to an in vitro endometrial three-dimensional cell culture model Hum. Reprod., November 1, 2007; 22(11): 3031 - 3037. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. V. DeSouza, J. Grigull, S. Ghanny, V. Dube, A. D. Romaschin, T. J. Colgan, and K. W. M. Siu Endometrial Carcinoma Biomarker Discovery and Verification Using Differentially Tagged Clinical Samples with Multidimensional Liquid Chromatography and Tandem Mass Spectrometry Mol. Cell. Proteomics, July 1, 2007; 6(7): 1170 - 1182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Seppala, R. N. Taylor, H. Koistinen, R. Koistinen, and E. Milgrom Glycodelin: A Major Lipocalin Protein of the Reproductive Axis with Diverse Actions in Cell Recognition and Differentiation Endocr. Rev., August 1, 2002; 23(4): 401 - 430. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Song, S. Ramaswamy, S. Ramachandran, L. C. Flowers, I. R. Horowitz, J. A. Rock, and S. Parthasarathy Angiogenic role for glycodelin in tumorigenesis PNAS, July 13, 2001; (2001) 151151198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Ghosh, P.G.L. Lalitkumar, V. J. Wong, A.G. Hendrickx, and J. Sengupta Preimplantation embryo morphology following early luteal phase anti-nidatory treatment with mifepristone (RU486) in the rhesus monkey Hum. Reprod., January 1, 2000; 15(1): 180 - 188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mukhopadhyay, S. Sundereshan, C. Rao, and A. A. Karande Placental Protein 14 Induces Apoptosis in T Cells but Not in Monocytes J. Biol. Chem., July 20, 2001; 276(30): 28268 - 28273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Song, S. Ramaswamy, S. Ramachandran, L. C. Flowers, I. R. Horowitz, J. A. Rock, and S. Parthasarathy Angiogenic role for glycodelin in tumorigenesis PNAS, July 31, 2001; 98(16): 9265 - 9270. [Abstract] [Full Text] [PDF]
|
|