Human Reproduction, Vol 13, 634-638, Copyright © 1998 by Oxford University Press
I Aslam and S Fishel
Testicular cell suspensions were prepared from obstructive and non-
obstructive azoospermic men and were cultured in vitro for 96 h as (i)
mixed cell populations and (ii) isolated homogeneous populations of primary
spermatocytes, round spermatids and elongating spermatids. The cells lost
their viability gradually during the first 24 h period. By 72 h almost 90%
of the cells were non-viable. Isolated pure fractions showed better
viability at each time interval (P < 0.0005). Throughout the culture
period primary spermatocytes, elongating spermatids and other
non-spermatogenic cells showed no change in their morphology, but almost
22% of round spermatids showed growth of flagella. Most of the round
spermatids developed their flagella during the first 4-8 h period of
culture. Isolated pure round spermatids showed better flagellar growth
compared with mixed cell suspensions (P < 0.0005). The spermatogenic
cells were successfully cryopreserved. However, when mixed spermatogenic
cell suspensions were cryopreserved, more cells lost their viability
compared with when isolated pure fractions were cryopreserved (P <
0.0005).
ARTICLES
Short-term in-vitro culture and cryopreservation of spermatogenic cells used for human in-vitro conception
CARE, The Park Hospital, Arnold, Nottingham, UK.
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