Human Reproduction, Vol 13, 676-681, Copyright © 1998 by Oxford University Press
Y Shoukir, D Chardonnens, A Campana, P Bischof and D Sakkas
Improved embryo culture protocols now render more feasible the possibility
of obtaining human blastocysts after in-vitro fertilization. In this study
we present: (i) results of blastocyst development from supernumerary
embryos after co-culture on green monkey kidney epithelial cells and (ii)
pregnancy rates after transfer of frozen blastocysts. In addition, we have
examined the influence of the day of blastocyst freezing and the day of
transfer after the luteinizing hormone (LH) peak on pregnancy and
implantation rates. Of 423 supernumerary embryos, 200 developed to the
blastocyst stage (47.3%). By days 5 and 6, 67% of the blastocysts had
reached the blastocyst stage, and were frozen, compared to 28.5% by day 7.
When we compared the cases where only blastocysts frozen on days 5 and 6
were transferred compared to those frozen and transferred on or after day 7
the pregnancy rates were 7/18 (38.9%) and 1/16 (6.2%) respectively. In
contrast, when we examined the influence of the day of transfer we found
that pregnancies were established from day 5 up to day 9 post LH peak.
Based on these results, we suggest that every attempt should be made to
increase the development rate of supernumerary embryos to the blastocyst
stage, as it appears that the quality of blastocysts transferred, as shown
in this study by rate of development, plays a more crucial role than the
timing of transfer.
ARTICLES
The rate of development and time of transfer play different roles in influencing the viability of human blastocysts
Clinic of Infertility and Gynaecological Endocrinology--WHO Collaborating Centre in Human Reproduction, Department of Obstetrics and Gynaecology, University Hospital of Geneva, Switzerland.
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