Human Reproduction, Vol 13, 841-846, Copyright © 1998 by Oxford University Press
KK Vihko, M Blauer, E Kujansuu, S Vilska, T Alback, R Tuimala, P Tuohimaa and R Punnonen
A recently developed immunoenzymometric assay for activin B has been
characterized further by measurement during ovarian stimulation and
pregnancy. The assay is based on a monoclonal anti-peptide antibody,
anti-betaB(101-115). In addition to quantitative analyses, the antibody has
been used for immunohistochemical localization of the activin betaB-
subunit in human term placenta. Serum samples obtained from patients
suffering from tubal factor infertility who were admitted for in-vitro
fertilization (IVF) treatment protocols or from patients with proven
fertility who were admitted for laparoscopic tubal ligation were collected.
The aim was to correlate serum activin B concentrations with other
parameters during IVF and with phases of the menstrual cycle. Serum samples
obtained from healthy pregnant volunteers were studied to correlate activin
B concentrations with clinical parameters. During the IVF treatment
protocols, activin B was detectable in all patients studied, and a
significant negative correlation was observed between serum activin B and
oestradiol concentrations. On the other hand, no significant difference was
observed in activin B concentrations when serum samples obtained from
patients at different phases of the menstrual cycle were compared, and low
concentrations of activin B were observed in the samples obtained from
these patients. During pregnancy, a positive correlation was observed
between serum activin B concentrations and gestational age. In
immunohistochemical analyses of human placental tissue obtained from
healthy parturients, the activin betaB-subunit was present in trophoblast,
amniotic epithelial and Hofbauer cells. The results suggest a potential
clinical application in female reproductive medicine for serum activin B
measurements.
ARTICLES
Activin B: detection by an immunoenzymometric assay in human serum during ovarian stimulation and late pregnancy
Medical School, Department of Obstetrics and Gynecology, Tampere University Hospital, Finland.
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