Human Reproduction, Vol 13, 896-900, Copyright © 1998 by Oxford University Press
S Lopes, A Jurisicova, JG Sun and RF Casper
The objective of this study was to evaluate the effect of the generation of
reactive oxygen species (ROS) on the integrity of the DNA of human
spermatozoa, and to determine if pretreatment with antioxidants can reduce
DNA damage. Samples were obtained from 47 men undergoing infertility
investigation. ROS were generated in the samples by the addition of
xanthine/xanthine oxidase (X/XO) with or without antioxidants. After
incubation at timed intervals (0-2 h) with X/XO, the percentage of
spermatozoa with DNA fragmentation was determined using the method of
TdT-mediated DNA end-labelling (TUNEL). Time intervals were selected to
mimic the clinical situation in which spermatozoa are held for a period of
time after swim-up while the oocytes are prepared for ICSI. A significant
increase in sperm DNA damage was evident when samples were incubated in the
presence of ROS for intervals of 1 and 2 h, but not when incubated with ROS
for <1 h (P = 0.0001). The addition of antioxidants significantly
decreased the amount of DNA damage induced by ROS generation (P < 0.04).
ROS can cause an increase in DNA fragmentation and pretreatment with
antioxidants can reduce DNA damage.
ARTICLES
Reactive oxygen species: potential cause for DNA fragmentation in human spermatozoa
Department of Obstetrics and Gynecology, University of Toronto, Toronto Center for Advanced Reproductive Technology, Ontario, Canada.
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