Human Reproduction, Vol 13, 927-935, Copyright © 1998 by Oxford University Press
J Smitz and R Cortvrindt
Conditioned media from single mouse ovarian follicles cultured from the
early preantral stage up to complete maturity were analysed for different
immunoreactive inhibin forms. The inhibin assays measured (i)
alpha-specific inhibin, as represented by a mix of 32 and and 57 kDa
inhibins, inhibin precursors, alpha-subunit and its precursors; (ii)
dimeric inhibin A; and (iii) dimeric inhibin B. The validity of these
assays for the measurement of mouse inhibin was established. All forms of
inhibin were secreted in culture media from the preantral follicle stage
onwards. Inhibin B was the most sensitive marker for proliferation of early
stage follicles, while inhibin A secretion became predominant at later
stages, when antral-like cavities were formed in granulosa cell masses.
Supplementation of standard culture medium with recombinant follicle
stimulating hormone appeared to be the predominant regulator of inhibin
secretion; addition of recombinant luteinizing hormone throughout the
culture period did not cause any major shifts in the expression of dimeric
inhibin or alpha-specific inhibin forms. In the absence of theca cells
during isolation and culture (as reflected by absence of oestrogen
secretion), follicles grew at a reduced rate, and produced lower inhibin
concentration in conditioned medium. These data suggest (i) that monitoring
of dimeric inhibins can provide useful markers of the growth and
differentiation of cultured follicles and (ii) that dimeric inhibins A and
B are secreted at an earlier stage in vitro than in vivo.
ARTICLES
Inhibin A and B secretion in mouse preantral follicle culture
Centre for Reproductive Medicine, University Hospital and Medical School, Dutch-speaking Brussels Free University, Belgium.
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