Human Reproduction, Vol 13, 944-952, Copyright © 1998 by Oxford University Press
S Yamada, H Fujiwara, N Kataoka, T Honda, T Nakayama, T Higuchi, T Mori and M Maeda
A monoclonal antibody (mAb), HCL-2, was raised which reacts with
apolipoprotein-B, and it was shown by immunohistology that HCL-2 can be
used to analyse the uptake of apolipoprotein-B by steroid-producing cells
in vivo. In this study we have investigated the dynamic utilization of low
density lipoprotein (LDL) in human ovary by immunohistological localization
of apolipoprotein-B and LDL receptors using HCL-2 and anti-LDL receptor
mAb. In antral follicles, including those of <1 mm in diameter, both
apolipoprotein-B and LDL receptors were localized to theca interna cells,
but not granulosa cells. In pre- ovulatory follicles, the LDL receptor was
expressed on all granulosa cells. Apolipoprotein-B was also detected in
granulosa cells located at the basal layer, suggesting that they utilize
LDL through the basal lamina before ovulation. In mid-luteal phase, large
luteal cells seemed to stain more intensely for apolipoprotein-B than did
small luteal cells, suggesting that large lutal cells are the main sites of
LDL utilization. In regressing corpora lutea, the expression of LDL
receptor was weak, and apolipoprotein-B was rarely detected. In corpora
lutea of early pregnancy, LDL receptor and apolipoprotein-B were localized
to both luteal cells. These findings show the precise dynamic changes in
LDL uptake by human ovarian cells during their differentiation in vivo.
ARTICLES
Stage-specific uptake of apolipoprotein-B in ovarian follicles and corpora lutea of the menstrual cycle and early pregnancy
Department of Gynecology and Obstetrics, Faculty of Medicine, Kyoto University, Japan.
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