Human Reproduction, Vol 13, 964-970, Copyright © 1998 by Oxford University Press
B Dale, Y Menezo, J Cohen, L DiMatteo and M Wilding
We have used fluorescence techniques to study the regulation of
intracellular pH during fertilization and preimplantation embryo
development in human oocytes. The intracellular pH of human oocytes during
maturation and fertilization was always 7.4, suggesting that these
processes do not involve long-term changes in intracellular pH. The
recovery of intracellular pH of human oocytes and embryos after
extracellular acid or alkaline shock was investigated. Fresh metaphase II
oocytes and preimplantation embryos showed similar rates of recovery
following alkaline shock. However, aged and immature oocytes had a
significantly slower rate of recovery to physiological pH. Early embryos up
to the morula stage were unable to recover following acidosis, whereas
blastocysts could control both acidosis and alkalosis. We assessed the
sensitivity of fertilization and early development in the human to
extracellular pH. Our results show that insemination in the human is
pH-sensitive, whereas intracytoplasmic injection (ICSI) activated oocytes
at all pH tested. We suggest that this is due to the pH-sensitivity of the
sperm-zona pellucida interaction, which is bypassed during the ICSI
procedure. Further development in human embryos is more sensitive to
alkalinity than acidity. We discuss these results in terms of the
extracellular pH in vivo in the female reproductive tract.
ARTICLES
Intracellular pH regulation in the human oocyte
Biologia della Riproduzione, Clinica Villa del Sole, Naples, Italy.
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