Human Reproduction, Vol 13, 987-990, Copyright © 1998 by Oxford University Press
A Neuer, C Mele, HC Liu, Z Rosenwaks and SS Witkin
Two-cell mouse embryos (B6D2F1) were cultured in the presence or absence of
100 microg/ml monoclonal antibodies specific for the mammalian 60 kDa
(HSP60), 70 kDa (HSP70) and 90 kDa (HSP90) heat shock proteins. Embryo
development was evaluated after 3, 5 and 7 days in culture by determining
the number of blastocysts, hatched blastocysts and outgrown trophoblasts at
the successive time points. At day 3, only 29% (22/75) of the embryos
cultured with anti-HSP60 antibody developed to the blastocyst stage (P <
0.0001) as compared to 67% (31/46) of the embryos cultured with anti-HSP70,
72% (43/60) cultured with anti-HSP90, and 79% (49/62) in medium plus mouse
IgG1. By day 5, hatched embryos were present in 28% (13/ 46) of the
cultures containing anti-HSP70 (P < 0.0001), as opposed to 57% (34/60)
containing anti-HSP90 and 73% (45/62) containing IgG1. At day 7, outgrown
trophoblasts were observed in 9% (4/46) of cultures containing anti-HSP70
(P < 0.0001), 45% (27/60) containing anti-HSP90 (P < 0.01) and 66%
(41/62) cultured in medium plus IgG1. Antibodies to different heat shock
proteins exerted a detrimental effect on mouse embryo development at unique
development stages. Immune sensitization to heat shock proteins may be a
cause of reproductive failure.
ARTICLES
Monoclonal antibodies to mammalian heat shock proteins impair mouse embryo development in vitro
Department of Obstetrics and Gynecology, Cornell University Medical College, New York, NY 10021, USA.
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