Human Reproduction, Vol 13, 1292-1302, Copyright © 1998 by Oxford University Press
R Cortvrindt, Y Hu and J Smitz
The effects of recombinant luteinizing hormone (rLH) and recombinant
follicle stimulating hormone (rFSH) both separately and together were
analysed on the in-vitro maturation of a well-defined class of mouse early
preantral follicles. Metaphase-II (MII) oocytes were only obtained when rLH
or rFSH or a combination of both hormones was added to a standard rich
culture medium at constant doses throughout the culture period. Without
gonadotrophins only 8% of the follicles reached day 12 of culture. Follicle
survival was optimal (between 90 and 100%) when rFSH was used;
significantly fewer follicles were kept intact when rLH was added as the
only supplement (54%; P < 0.001). Theca cells played a fundamental role
in follicle survival when rLH was the only supplement. Presence of rLH
significantly influenced the formation of antral-like cavities: on day 12,
59% of follicles cultured with rLH + rFSH had a large cavity, as compared
with only 21% in cultures with rFSH only (P < 0.0001). The rHCG
stimulated germinal vesicle breakdown rate from the different
gonadotrophin-supplemented cultures on day 12 was not significantly
different (FSH: 89%; LH: 70%; FSH/LH: 83%). However, addition of rLH to
rFSH did significantly improve (P < 0.05) the completion of the first
meiotic division up to the MII stage (FSH: 46%; LH: 39%; FSH/LH: 76%).
Interfollicle differences in steroid production were very large and
depended on the presence of theca cells and on the kind of gonadotrophin
supplemented. Without theca cells, oestrogen concentrations in
rFSH-supplemented cultures were 100 times lower than their theca-containing
counterparts during the entire culture period. From culture day 9 onwards
most conditioned media had increased basal progesterone concentrations.
This study points up the in-vitro role of LH on the differentiation of
follicle-like structures and the oocyte's meiotic maturation and suggests
an important role for theca cells in providing modulators of in-vitro
gonadotrophin action.
ARTICLES
Recombinant luteinizing hormone as a survival and differentiation factor increases oocyte maturation in recombinant follicle stimulating hormone-supplemented mouse preantral follicle culture
Centre for Reproductive Medicine, University Hospital and Medical School, Dutch-speaking Brussels Free University, Belgium.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Xu, E. R. West-Farrell, R. L. Stouffer, L. D. Shea, T. K. Woodruff, and M. B. Zelinski Encapsulated Three-Dimensional Culture Supports Development of Nonhuman Primate Secondary Follicles Biol Reprod, September 1, 2009; 81(3): 587 - 594. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Honda, M. Hirose, K. Hara, S. Matoba, K. Inoue, H. Miki, H. Hiura, M. Kanatsu-Shinohara, Y. Kanai, T. Kono, et al. Isolation, characterization, and in vitro and in vivo differentiation of putative thecal stem cells PNAS, July 24, 2007; 104(30): 12389 - 12394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Xu, E. West, L. D. Shea, and T. K. Woodruff Identification of a Stage-Specific Permissive In Vitro Culture Environment for Follicle Growth and Oocyte Development Biol Reprod, December 1, 2006; 75(6): 916 - 923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. K. Kreeger, N. N. Fernandes, T. K. Woodruff, and L. D. Shea Regulation of Mouse Follicle Development by Follicle-Stimulating Hormone in a Three-Dimensional In Vitro Culture System Is Dependent on Follicle Stage and Dose Biol Reprod, November 1, 2005; 73(5): 942 - 950. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, H. Newton, J. A. Spaliviero, C. M. Allan, B. Marshan, D. J. Handelsman, and P. J. Illingworth Gonadotropin Control of Inhibin Secretion and the Relationship to Follicle Type and Number in the hpg Mouse Biol Reprod, October 1, 2005; 73(4): 610 - 618. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bjercke, P. Fedorcsak, T. Abyholm, R. Storeng, G. Ertzeid, N. Oldereid, A. Omland, and T. Tanbo IVF/ICSI outcome and serum LH concentration on day 1 of ovarian stimulation with recombinant FSH under pituitary suppression Hum. Reprod., September 1, 2005; 20(9): 2441 - 2447. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I Demeestere, J Centner, C Gervy, Y Englert, and A Delbaere Impact of various endocrine and paracrine factors on in vitro culture of preantral follicles in rodents Reproduction, August 1, 2005; 130(2): 147 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Adriaens, R. Cortvrindt, and J. Smitz Differential FSH exposure in preantral follicle culture has marked effects on folliculogenesis and oocyte developmental competence Hum. Reprod., February 1, 2004; 19(2): 398 - 408. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Sun, I. Betzendahl, Y. Shen, R. Cortvrindt, J. Smitz, and U. Eichenlaub-Ritter Preantral follicle culture as a novel in vitro assay in reproductive toxicology testing in mammalian oocytes Mutagenesis, January 1, 2004; 19(1): 13 - 25. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Grondahl, J. Breinholt, P. Wahl, A. Murray, T. H. Hansen, I. Faerge, C. E. Stidsen, K. Raun, and C. Hegele-Hartung Physiology of meiosis-activating sterol: endogenous formation and mode of action Hum. Reprod., January 1, 2003; 18(1): 122 - 129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Demeestere, A. Delbaere, C. Gervy, M. Van den Bergh, F. Devreker, and Y. Englert Effect of preantral follicle isolation technique on in-vitro follicular growth, oocyte maturation and embryo development in mice Hum. Reprod., August 1, 2002; 17(8): 2152 - 2159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Balasch, E. Vidal, J. Penarrubia, R. Casamitjana, F. Carmona, M. Creus, F. Fabregues, and J. A. Vanrell Suppression of LH during ovarian stimulation: analysing threshold values and effects on ovarian response and the outcome of assisted reproduction in down-regulated women stimulated with recombinant FSH Hum. Reprod., August 1, 2001; 16(8): 1636 - 1643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hu, I. Betzendahl, R. Cortvrindt, J. Smitz, and U. Eichenlaub-Ritter Effects of low O2 and ageing on spindles and chromosomes in mouse oocytes from pre-antral follicle culture Hum. Reprod., April 1, 2001; 16(4): 737 - 748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wu, B. R. Emery, and D. T. Carrell In Vitro Growth, Maturation, Fertilization, and Embryonic Development of Oocytes from Porcine Preantral Follicles Biol Reprod, January 1, 2001; 64(1): 375 - 381. [Abstract] [Full Text]
|
|
|
|
|
|
J. Wu, P. L. Nayudu, P. S. Kiesel, and H. W. Michelmann Luteinizing Hormone Has a Stage-Limited Effect on Preantral Follicle Development In Vitro Biol Reprod, July 1, 2000; 63(1): 320 - 327. [Abstract] [Full Text]
|
|
|
|
|
|
C. Anderiesz, A.-P. Ferraretti, C. Magli, A. Fiorentino, D. Fortini, L. Gianaroli, G.M. Jones, and A.O. Trounson Effect of recombinant human gonadotrophins on human, bovine and murine oocyte meiosis, fertilization and embryonic development in vitro Hum. Reprod., May 1, 2000; 15(5): 1140 - 1148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Hovatta, C. Wright, T. Krausz, K. Hardy, and R. M.L. Winston Human primordial, primary and secondary ovarian follicles in long-term culture: effect of partial isolation Hum. Reprod., October 1, 1999; 14(10): 2519 - 2524. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hayashi, E. A. McGee, G. Min, C. Klein, U. M. Rose, M. v. Duin, and A. J. W. Hsueh Recombinant Growth Differentiation Factor-9 (GDF-9) Enhances Growth and Differentiation of Cultured Early Ovarian Follicles Endocrinology, March 1, 1999; 140(3): 1236 - 1244. [Abstract] [Full Text]
|
|