Human Reproduction, Vol 13, 1455-1460, Copyright © 1998 by Oxford University Press
I Foldesi, M Breckwoldt and J Neulen
In this study the effect of recombinant human follicle stimulating hormone
(rFSH) on oestradiol production by human granulosa-lutein cells was
examined in long-term culture, in the presence or absence of androgens.
Cells were harvested at the time of follicular aspiration after ovarian
hyperstimulation for in-vitro fertilization and cultured for 9 days.
Granulosa cells were capable of secreting oestradiol spontaneously even
without androgen and gonadotrophin support. Basal oestradiol secretion was
relatively high and variable (421.3 +/- 159.3 pg/ml, mean +/- SEM, n = 13)
on the first day and decreased gradually to 16.7 +/- 3.1 pg/ml on day 9.
Addition of androgens (testosterone or androstenedione) to the incubation
medium enhanced dose-dependently basal oestrogen production on days 5, 7
and 9. The androgen/oestrogen conversion rate remained constantly high
during the culture, even without rFSH. After pre-incubation for 3 days,
addition of rFSH resulted in a dose- and time-dependent increase in
granulosa-lutein cell oestrogen production in the absence of exogenous
androgens. Testosterone supplementation caused considerably higher basal
oestradiol concentrations, however rFSH failed to further stimulate the
oestrogen release from granulosa-lutein cells, suggesting that these cells
cultured in vitro possess high aromatase activity even without rFSH
support.
ARTICLES
Oestradiol production by luteinized human granulosa cells: evidence of the stimulatory action of recombinant human follicle stimulating hormone
Department of Obstetrics and Gynaecology, University of Freiburg, Germany.
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