Human Reproduction, Vol 13, 1898-1900, Copyright © 1998 by Oxford University Press
R Bernabeu, N Cremades, K Takahashi and M Sousa
We present nine cases of spermatid intracytoplasmic injection for the
treatment of non-obstructive azoospermia. In eight cases, no elongated
spermatids or spermatozoa were found in previous spermiograms or testicular
biopsies. In these patients, treatment was performed using ejaculated (n =
6) and testicular (n = 2) retrieved round spermatids (Sa type). In cases
where ejaculated round spermatids were used, they were isolated on the day
before oocyte retrieval and left in culture for 24 h before
intracytoplasmic sperm injection (ICSI). No pregnancy was obtained in
either group, although culturing seemed to increase the fertilization rate.
In one other case, elongated spermatids were observed in the previous
spermiogram and thus a normal ICSI procedure was scheduled. However, on the
day of oocyte retrieval, no spermatids could be recovered from fresh
sequential ejaculates, and a testicular open biopsy was then performed.
Both round and elongated spermatids were found in the testicular tissue,
but only the more mature germinal cells (Sb2) were injected. From this
case, a normal pregnancy was obtained which resulted in the birth by
Caesarean section at 37 weeks of gestation of a normal healthy baby girl,
weighing 2700 g.
ARTICLES
Successful pregnancy after spermatid injection
Instituto Bernabeu de Fertilidad y Ginecologia, Alicante, Spain.
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