Human Reproduction, Vol 13, 1922-1927, Copyright © 1998 by Oxford University Press
D Hlinka, M Herman, J Vesela, R Hredzak, S Horvath and J Pacin
In a controlled study we compared the outcome of intracytoplasmic sperm
injection (ICSI) performed by two different methods. The oocytes from 20
patients were equally divided into two groups and injected either by
conventional ICSI using polyvinylpyrrolidone (PVP) or by a modified PVP-
free ICSI procedure. While in the conventional ICSI method the spermatozoon
is aspirated into the injection pipette, in the modified ICSI procedure the
spermatozoon is attached to the end of the narrow micropipette by
aspirating its tail. The sperm head is never drawn into the pipette.
Accordingly, even a fast-moving spermatozoon can be 'caught' easily. As a
result of such an aspiration the spermatozoon loses its motility.
Therefore, PVP is required neither to slow down the movement of the
spermatozoon nor to facilitate the movement of the solution in the
injection pipette. A total of 230 mature oocytes were injected by both
methods and the results were analysed. No differences were observed in
survival rate between the two ICSI procedures (89% and 91%, respectively).
However, the proportion of normally fertilized oocytes was significantly
higher after microfertilization by modified ICSI (74%) when compared with
the outcome of the conventional ICSI method (62%). The frequency of
abnormal fertilization was not influenced by the method of ICSI used. The
cleavage rate and quality of resulting embryos were also comparable. In
conclusion, we have demonstrated a modified ICSI method which does not
require the use of PVP. When compared with the conventional ICSI procedure,
even better fertilization rates can be achieved. The proposed ICSI
modification may provide an alternative procedure for elimination of the
potentially harmful effects which may be associated with conventional ICSI.
ARTICLES
A modified method of intracytoplasmic sperm injection without the use of polyvinylpyrrolidone
2nd Clinic of Gynaecology and Obstetrics, University Hospital of L. Pasteur, Kosice, Slovak Republic.
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