Skip Navigation

This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (60)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Spano, M.
Right arrow Articles by Bonde, J. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spano, M.
Right arrow Articles by Bonde, J. P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol 13, 2495-2505, Copyright © 1998 by Oxford University Press

ARTICLES

The applicability of the flow cytometric sperm chromatin structure assay in epidemiological studies. Asclepios

M Spano, AH Kolstad, SB Larsen, E Cordelli, G Leter, A Giwercman and JP Bonde
Section of Toxicology and Biomedical Sciences, ENEA CR Casaccia, Rome, Italy.

The impact of demographic, lifestyle, and seminal factors on the sperm chromatin structure assay (SCSA) parameters was evaluated in a population of 277 healthy Danish men. This cohort was established within the framework of a European Concerted Action on occupational hazards to male reproductive capability in order to examine the possible reproductive effects of exposure to styrene or pesticides. The SCSA measures the susceptibility of sperm DNA to in-situ acid-induced denaturation, by multiparameter flow cytometric analysis after staining with the DNA-specific fluorescent dye acridine orange. The green versus red bivariate cytogram patterns were quite variable among donors, showing a wide heterogeneity of sperm DNA denaturability. Nevertheless, in those cases where we had the possibility to measure two semen samples from the same donor, the cytogram pattern remained stable over time (0.64 < r < 0.78). Analysis of variance demonstrated that the SCSA results can be influenced by the age of the donor (P < 0.0001), smoking habits (P < 0.05), the presence of leukocytes and immature germ forms in the ejaculate (P < 0.0001), and the duration of sexual abstinence (P < 0.0001). Furthermore, the relationship between the SCSA data and sperm concentration, morphology, and vitality was weak (-0.22 < r < - 0.46). Therefore, the SCSA provides independent and complementary measurements of semen quality and is thus a useful tool for epidemiological studies, but the effects of some confounders should be accounted for in the survey design and analysis.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
C. de Jager, N.H. Aneck-Hahn, M.S. Bornman, P. Farias, G. Leter, P. Eleuteri, M. Rescia, and M. Spano
Sperm chromatin integrity in DDT-exposed young men living in a malaria area in the Limpopo Province, South Africa
Hum. Reprod., October 1, 2009; 24(10): 2429 - 2438.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
R. D. van Beek, M. Smit, M. M. van den Heuvel-Eibrink, F. H. de Jong, F. G. Hakvoort-Cammel, C. van den Bos, H. van den Berg, R. F.A. Weber, R. Pieters, and S. M.P.F. de Muinck Keizer-Schrama
Inhibin B is superior to FSH as a serum marker for spermatogenesis in men treated for Hodgkin's lymphoma with chemotherapy during childhood
Hum. Reprod., December 1, 2007; 22(12): 3215 - 3222.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
M. Long, A. Stronati, D. Bizzaro, T. Kruger, G.-C. Manicardi, P. S Hjelmborg, M. Spano, A. Giwercman, G. Toft, J. P. Bonde, et al.
Relation between serum xenobiotic-induced receptor activities and sperm DNA damage and sperm apoptotic markers in European and Inuit populations
Reproduction, February 1, 2007; 133(2): 517 - 530.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
T.E. Schmid, B. Eskenazi, A. Baumgartner, F. Marchetti, S. Young, R. Weldon, D. Anderson, and A.J. Wyrobek
The effects of male age on sperm DNA damage in healthy non-smokers
Hum. Reprod., January 1, 2007; 22(1): 180 - 187.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
A Stronati, G C Manicardi, M Cecati, M Bordicchia, L Ferrante, M Spano, G Toft, J P Bonde, B A G Jonsson, A Rignell-Hydbom, et al.
Relationships between sperm DNA fragmentation, sperm apoptotic markers and serum levels of CB-153 and p,p'-DDE in European and Inuit populations.
Reproduction, December 1, 2006; 132(6): 949 - 958.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
O. Stahl, J. Eberhard, K. Jepson, M. Spano, M. Cwikiel, E. Cavallin-Stahl, and A. Giwercman
Sperm DNA integrity in testicular cancer patients
Hum. Reprod., December 1, 2006; 21(12): 3199 - 3205.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. J. Wyrobek, B. Eskenazi, S. Young, N. Arnheim, I. Tiemann-Boege, E. W. Jabs, R. L. Glaser, F. S. Pearson, and D. Evenson
Advancing age has differential effects on DNA damage, chromatin integrity, gene mutations, and aneuploidies in sperm
PNAS, June 20, 2006; 103(25): 9601 - 9606.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
T. Said, A. Agarwal, S. Grunewald, M. Rasch, T. Baumann, C. Kriegel, L. Li, H.-J. Glander, A. J. Thomas Jr., and U. Paasch
Selection of Nonapoptotic Spermatozoa As a New Tool for Enhancing Assisted Reproduction Outcomes: An In Vitro Model
Biol Reprod, March 1, 2006; 74(3): 530 - 537.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Spano, G. Toft, L. Hagmar, P. Eleuteri, M. Rescia, A. Rignell-Hydbom, E. Tyrkiel, V. Zvyezday, J.P. Bonde, and INUENDO
Exposure to PCB and p, p'-DDE in European and Inuit populations: impact on human sperm chromatin integrity
Hum. Reprod., December 1, 2005; 20(12): 3488 - 3499.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Bungum, P. Humaidan, M. Spano, K. Jepson, L. Bungum, and A. Giwercman
The predictive value of sperm chromatin structure assay (SCSA) parameters for the outcome of intrauterine insemination, IVF and ICSI
Hum. Reprod., June 1, 2004; 19(6): 1401 - 1408.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Loft, T. Kold-Jensen, N. H. Hjollund, A. Giwercman, J. Gyllemborg, E. Ernst, J. Olsen, T. Scheike, H. E. Poulsen, and J. P. Bonde
Oxidative DNA damage in human sperm influences time to pregnancy
Hum. Reprod., June 1, 2003; 18(6): 1265 - 1272.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. Richthoff, M. Spano, Y.L. Giwercman, B. Frohm, K. Jepson, J. Malm, S. Elzanaty, M. Stridsberg, and A. Giwercman
The impact of testicular and accessory sex gland function on sperm chromatin integrity as assessed by the sperm chromatin structure assay (SCSA)
Hum. Reprod., December 1, 2002; 17(12): 3162 - 3169.
[Abstract] [Full Text] [PDF]

Home page
 Occup. Environ. Med.Home page
J P Bonde, M Joffe, P Apostoli, A Dale, P Kiss, M Spano, F Caruso, A Giwercman, L Bisanti, S Porru, et al.
Sperm count and chromatin structure in men exposed to inorganic lead: lowest adverse effect levels
Occup. Environ. Med., April 1, 2002; 59(4): 234 - 242.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
W. Kuczynski, M. Dhont, C. Grygoruk, D. Grochowski, S. Wolczynski, and M. Szamatowicz
The outcome of intracytoplasmic injection of fresh and cryopreserved ejaculated spermatozoa--a prospective randomized study
Hum. Reprod., October 1, 2001; 16(10): 2109 - 2113.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. Molina, J.A. Castilla, J.L. Castano, J. Fontes, N. Mendoza, and L. Martinez
Chromatin status in human ejaculated spermatozoa from infertile patients and relationship to seminal parameters
Hum. Reprod., March 1, 2001; 16(3): 534 - 539.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Reichart, I. Kahane, and B. Bartoov
In Vivo and In Vitro Impairment of Human and Ram Sperm Nuclear Chromatin Integrity by Sexually Transmitted Ureaplasma urealyticum Infection
Biol Reprod, October 1, 2000; 63(4): 1041 - 1048.
[Abstract] [Full Text]

Home page
 Hum ReprodHome page
M. Sergerie, S. Ouhilal, F. Bissonnette, J. Brodeur, and G. Bleau
Lack of association between smoking and DNA fragmentation in the spermatozoa of normal men
Hum. Reprod., June 1, 2000; 15(6): 1314 - 1321.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A.D. Esterhuizen, D.R. Franken, J.G.H. Lourens, E. Prinsloo, and L.H. van Rooyen
Sperm chromatin packaging as an indicator of in-vitro fertilization rates
Hum. Reprod., March 1, 2000; 15(3): 657 - 661.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Gergely, E. Kovanci, L. Senturk, E. Cosmi, L. Vigue, and G. Huszar
Morphometric assessment of mature and diminished-maturity human spermatozoa: sperm regions that reflect differences in maturity
Hum. Reprod., August 1, 1999; 14(8): 2007 - 2014.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
K.L. Larson, J.D. Brannian, B.K. Timm, L.K. Jost, and D.P. Evenson
Density gradient centrifugation and glass wool filtration of semen remove spermatozoa with damaged chromatin structure
Hum. Reprod., August 1, 1999; 14(8): 2015 - 2019.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
D.P. Evenson, L.K. Jost, D. Marshall, M.J. Zinaman, E. Clegg, K. Purvis, P. de Angelis, and O.P. Claussen
Utility of the sperm chromatin structure assay as a diagnostic and prognostic tool in the human fertility clinic
Hum. Reprod., April 1, 1999; 14(4): 1039 - 1049.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
M. Spano, E. Cordelli, G. Leter, F. Lombardo, A. Lenzi, and L. Gandini
Nuclear chromatin variations in human spermatozoa undergoing swim-up and cryopreservation evaluated by the flow cytometric sperm chromatin structure assay
Mol. Hum. Reprod., January 1, 1999; 5(1): 29 - 37.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.