Human Reproduction, Vol 13, 2561-2563, Copyright © 1998 by Oxford University Press
J Wu, L Zhang and P Liu
This study aimed to investigate the development of human preantral
follicles and oocyte maturation in vitro. Preantral follicles were obtained
from follicular aspirates during egg retrieval carried out during an
in-vitro fertilization (IVF) programme. They were first incubated in Ham's
F10 medium with 15% fetal cord serum (FCS). After 28 days, the medium was
supplemented with different doses of human menopausal gonadotrophin (HMG),
human follicular fluid (hFF) and epidermal growth factor (EGF) by
orthogonal design. Promotion of final maturation was completed in the
presence of HMG and hFF. Development from preantral to antral follicles was
found within 6-12 days of culture. With time, the proportion of follicles
with diameters of >300 microm increased at 21-28 days of culture (P <
0.005). The maximum number of oocytes extruded, and first polar body
formation, occurred in the presence of 0.15 IU/ml HMG 40% (v/v) hFF and 6
ng/ml EGF. We conclude that follicular aspirates obtained during egg
retrieval in an IVF programme contain many preantral follicles which could
develop into antral follicles with extrusion of oocytes in culture, and
that the oocytes can mature in vitro. Hence, a new source of human oocytes
is available.
ARTICLES
A new source of human oocytes: preliminary report on the identification and maturation of human preantral follicles from follicular aspirates
Department of Obstetrics and Gynecology, Third Clinical Medical College, Beijing Medical University, China.
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