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Human Reproduction, Vol. 13, No. suppl_4, pp. 109-116, 1998
© 1998 European Society of Human Reproduction and Embryology

Cryopreservation of activated mouse oocytes and zygote reconstitution after thaw

Jacob Levron1,2, Steen M. Willadsen3, Tim Shimmel1 and Jacques Cohen1,4

1 The Gamete and Embryo Research Laboratory, The Institute of Reproductive Medicine and Science of Saint Barnabas Medical Center NJ, USA 2 Department of Obstetrics and Gynecology, Sheba Medical Center Tel Hashomer, Israel 3 IVF Center, Sand Lake Hospital Orlando, USA

Correspondence: 4To whom correspondence should be addressed

A new approach to cryopreservation of unfertilized oocytes is proposed using techniques of artificial egg activation combined with nuclear transplantation. Matured mouse oocytes were released from metaphase II arrest by brief exposure to alcohol, allowed to progress to the pronuclear stage and then frozen according to a standard freezing protocol in propandiol. After thaw the female pronuclei were enucleated and fused with a male karyoplasts that were divided from in-vivo fertilized zygotes. Reconstituted zygotes, fresh and cryopreserved culture control zygotes were cultured to the blastocyst stage and transferred to pseudopregnant recipients. The rate of blastocyst formation was 75.8, 91.6 and 44.1% respectively. A total of 110, 215 and 70 blastocysts were transferred to pseudopregnant females respectively. The implantation rates were 36.4, 72.0 and 75.7% while the rates of fetal viability at mid-gestation were 15.5 (P < 0.0001), 51.1 and 37.1% respectively.

Key words: artificial egg activation/cryopreservation/nuclear transplantation/zygote reconstitution


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