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Human Reproduction, Vol. 13, No. suppl_4, pp. 212-217, 1998
© 1998 European Society of Human Reproduction and Embryology

Randomized comparison of three media used for embryo culture after intracytoplasmic sperm injection

Jean Parinaud1,3, Anna Veiga2, Gérard Vieitez1, Anna Busquets2, Pierrette Milhet1, Gloria Calderon2, Gérard Richoilley1 and Pedro Barri2

1 Laboratoire de Fécondation In Vitro CHU La Grave, 31052 Toulouse Cedex, France 2 Servici de Medicina de la Reproductio, Departamento de Obstetricia y Ginecologia, Institut Universitari Dexeus Paseo Bonanova 67, 08017 Barcelona, Spain

Correspondence: 3To whom correspondence should be addressed

Since the metabolic requirements of fertilization and early embryonic development are very different, we have tested a new culture medium (EllioStep2, Ellios Bio-Media, Paris, France) specially designed for the first cleavages and compared it with two conventional media: BM1 (Ellios Bio-Media, Paris, France) and IVF50 (Scandinavian IVF Science, Gothenburg, Sweden). In order to avoid any interference with fertilization, the test was performed as part of an intracytoplasmic sperm injection (ICSI) study. A total of 416 ICSI attempts were randomly performed using one or other of the media. After sperm injection, oocytes were incubated either in EllioStep2 or in BM1 or in IVF50. The embryo quality, pregnancy and implantation rates, number of frozen embryos were compared in the different media. The percentage of fair embryos (grades 4 and 3) was significantly higher when EllioStep2 was used than when oocytes was cultured in BM1 medium (54 versus 47%; P < 0.01) or in IVF50 (69 versus 61%; P < 0.01). The pregnancy rate per transfer and the implantation rate were not significantly higher with EllioStep2 than with BM1 or IVF50. However, the percentage of embryo freezings per attempt was significantly higher with EllioStep2 than with BM1 (47/105 versus 28/105; P < 0.01). In conclusion, the use of EllioStep2 is associated with an increase in embryo quality permitting a higher number of embryo freezings.

Key words: culture medium/embryo/ICSI


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