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Human Reproduction, Vol. 13, No. suppl_4, pp. 61-70, 1998
© 1998 European Society of Human Reproduction and Embryology

The first births and ongoing pregnancies associated with sperm cryopreservation within evacuated egg zonae

Reneé Walmsley, Jacques Cohen1, Toni Ferrara-Congedo, Adrienne Reing and John Garrisi

The Institute of Reproductive Medicine and Science of Saint Barnabas Medical Center 101 Old Short Hills Road, Suite 501, West Orange, NJ 07052, USA

Correspondence: 1To whom correspondence should be addressed

This new procedure principally aims to avoid a second or possibly multiple surgical procedures for sperm extraction from the male partner in cases of limited amounts of sperm cells, where normal freeze–thaw protocols would fail. Patients (n = 34) diagnosed as azoospermic, extreme oligozoospermic, or oligoasthenozoospermic underwent the process of sperm cryopreservation within evacuated egg zonae. Other samples were allocated to conventional sperm freezing. Sperm samples were acquired using testicular sperm extraction (TESE), microepididymal sperm aspiration (MESA), or fresh ejaculate. Subsequently, five of these 34 couples have undergone in-vitro fertilization (IVF) and achieved normal fertilization using post-thawed spermatozoa frozen under zonae pellucidae in conjunction with intracytoplasmic sperm injection (ICSI). The average fertilization rate for the post-thaw injected spermatozoa was 65%. This is comparable with the regular fertilization rate of 65% for combined MESA and TESE using fresh spermatozoa. All patients underwent embryo transfer. The average implantation rate per embryo was 31%; nearly the same for regular MESA/TESE ICSI cycles (32%). The first pregnancy associated with this procedure concluded with the full term delivery of healthy twin girls on July 18, 1997. The remaining four thaw procedures resulted in another twin delivery, an ongoing singleton gestation, a negative pregnancy test and a biochemical pregnancy respectively.

Key words: azoospermia/evacuated zonae/MESA/severe male factor/TESE


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 Hum Reprod UpdateHome page
F. AbdelHafez, M. Bedaiwy, S. A. El-Nashar, E. Sabanegh, and N. Desai
Techniques for cryopreservation of individual or small numbers of human spermatozoa: a systematic review
Hum. Reprod. Update, March 1, 2009; 15(2): 153 - 164.
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