Human Reproduction, Vol. 14, No. 1, 55-59,
January 1999
© 1999 European Society of Human Reproduction and Embryology
The mouse as a model to study adhesion formation following endoscopic surgery: a preliminary report
1 Centre for Surgical Technologies (CHT) and 2 Department of Obstetrics and Gynaecology, University Hospital Gasthuisberg, K. U. Leuven, Minderbroederstraat 17, B-3000, Leuven, Belgium
Our aim was to investigate the feasibility of a mouse model to study adhesion formation following endoscopic surgery. Following preliminary studies to establish anaesthesia and pneumoperitoneum pressure, a prospective randomized study was carried out to investigate the effect of CO2 pneumoperitoneum on postoperative adhesions. In group I (control group), the duration of pneumoperitoneum was shorter than 5 min. In groups II, III and IV, pneumoperitoneum was maintained for 60 min without flow, with a continuous low flow (1 ml/min) and a continuous high flow (10 ml/min) through the abdominal cavities of the mice using non-humidified CO2, respectively. Adhesions were scored after 7 days by laparotomy. The total adhesion scores were 0.9 ± 0.8 (n = 15) in control group, 2.4 ± 0.8 (n = 15) (P < 0.001 versus control group) in group II with no flow, 2.6 ± 1.3 (n = 15) (P < 0.001 versus control group) in group III with a continuous low flow and 4.3 ± 0.9 (n = 15) (P < 0.001 versus control group and P < 0.001 versus group II and III) in group IV with a continuous high flow. In conclusion, the mouse can be used as a model to study adhesion formation following endoscopic surgery. Duration of CO2 pneumoperitoneum is a co-factor in adhesion formation.
Key words: adhesions/endoscopy/mouse
Presented in part at the International Congress of Peritoneal Tissue Healing, the 4th Peritoneum and Peritoneal Access Meeting, in Göteborg (Gothenburg), Sweden, September 17–19, 1997
3 To whom correspondence should be addressed
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