Human Reproduction, Vol. 14, No. 10, 2570-2574,
October 1999
© 1999 European Society of Human Reproduction and Embryology
The influence of in-vitro culture versus stimulated and untreated oviductal environment on mouse embryo development and implantation
1 Leuven University Fertility Center, 2 Experimental Laboratory of Obstetrics and Gynecology, and 3 Division of Endoscopic Surgery, Department of Obstetrics and Gynecology, University Hospital Gasthuisberg, Catholic University Leuven, Herestraat 49, B-3000 Leuven, Belgium
A prospective randomised study was performed to evaluate stimulated versus natural oviductal environment in comparison with in-vitro culture for the developmental capacity of mouse embryos. Therefore, embryos of superovulated F1 hybrid CBAxC57Bl females were collected at 17, 22, 41 and 46 h after human chorionic gonadotrophin treatment and randomly divided into five groups. They were either transferred immediately to untreated pseudopregnant females, cultured in vitro for 5, 24 or 29 h before transfer, or cultured in vitro for 96 h to blastocysts. The transfers resulted in an impaired implantation (P < 0.001) and a lower numbers of living fetuses (P < 0.001) when embryos had been exposed longer to the stimulated oviductal environment. Similar results were obtained after a longer period of in-vitro culture (P < 0.05). However when embryos were flushed earlier from the superovulated mice and cultured longer in-vitro until the transfer was performed, the implantation rate was improved (P < 0.01). Blastocyst development, however, was better (P < 0.001) when embryos were flushed later. In conclusion, the stimulated oviductal environment impairs the developmental capacity of embryos in comparison with untreated pseudopregnant females. In-vitro culture is also suboptimal but better than the stimulated oviductal environment.
Key words: implantation/in-vitro culture/mouse embryo development/natural cycle/superovulation
4 To whom correspondence should be addressed
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  F. Guerif, M. Lemseffer, R. Bidault, O. Gasnier, M.H. Saussereau, V. Cadoret, C. Jamet, and D. Royere Single Day 2 embryo versus blastocyst-stage transfer: a prospective study integrating fresh and frozen embryo transfers Hum. Reprod., May 1, 2009; 24(5): 1051 - 1058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Perez-Crespo, P. Moreira, B. Pintado, and A. Gutierrez-Adan Factors From Damaged Sperm Affect Its DNA Integrity and Its Ability to Promote Embryo Implantation in Mice J Androl, January 1, 2008; 29(1): 47 - 54. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Kelley, K. L. Kind, M. Lane, R. L. Robker, J. G. Thompson, and L. J. Edwards Recombinant human follicle-stimulating hormone alters maternal ovarian hormone concentrations and the uterus and perturbs fetal development in mice Am J Physiol Endocrinol Metab, October 1, 2006; 291(4): E761 - E770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Tielens, B Verhasselt, J Liu, M Dhont, J Van Der Elst, and M Cornelissen Generation of embryonic stem cell lines from mouse blastocysts developed in vivo and in vitro: relation to Oct-4 expression Reproduction, July 1, 2006; 132(1): 59 - 66. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.M. Sibug, J. de Koning, A.M.I. Tijssen, M.C. de Ruiter, E.R. de Kloet, and F.M. Helmerhorst Urinary gonadotrophins but not recombinant gonadotrophins reduce expression of VEGF120 and its receptors flt-1 and flk-1 in the mouse uterus during the peri-implantation period Hum. Reprod., March 1, 2005; 20(3): 649 - 656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zudova, A. J. Wyrobek, J. Bishop, and F. Marchetti Impaired fertility in T-stock female mice after superovulation Reproduction, November 1, 2004; 128(5): 573 - 581. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. P. Fleming, W. Y. Kwong, R. Porter, E. Ursell, I. Fesenko, A. Wilkins, D. J. Miller, A. J. Watkins, and J. J. Eckert The Embryo and Its Future Biol Reprod, October 1, 2004; 71(4): 1046 - 1054. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. F. M. Mitwally and R. F. Casper Aromatase Inhibition Reduces the Dose of Gonadotropin Required for Controlled Ovarian Hyperstimulation Reproductive Sciences, September 1, 2004; 11(6): 406 - 415. [Abstract] [PDF]
|
|
|
|
|
|
M.F.M. Mitwally and R.F. Casper Aromatase inhibition reduces gonadotrophin dose required for controlled ovarian stimulation in women with unexplained infertility Hum. Reprod., August 1, 2003; 18(8): 1588 - 1597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.M. Sibug, F.M. Helmerhorst, A.M.I. Tijssen, E.R. de Kloet, and J. de Koning Gonadotrophin stimulation reduces VEGF120 expression in the mouse uterus during the peri-implantation period Hum. Reprod., June 1, 2002; 17(6): 1643 - 1648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Van der Auwera and T. D'Hooghe Superovulation of female mice delays embryonic and fetal development Hum. Reprod., June 1, 2001; 16(6): 1237 - 1243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Ertzeid and R. Storeng The impact of ovarian stimulation on implantation and fetal development in mice Hum. Reprod., February 1, 2001; 16(2): 221 - 225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Liu, J. Van der Elst, R. Van den Broecke, and M. Dhont Live Offspring by In Vitro Fertilization of Oocytes from Cryopreserved Primordial Mouse Follicles after Sequential In Vivo Transplantation and In Vitro Maturation Biol Reprod, January 1, 2001; 64(1): 171 - 178. [Abstract] [Full Text]
|
|