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Human Reproduction, Vol. 14, No. 5, 1182-1189, May 1999
© 1999 European Society of Human Reproduction and Embryology

Aromatase activity of human granulosa cells in vitro: effects of gonadotrophins and follicular fluid

P. Guet1,3, D. Royère1,4, A. Paris2, J. Lansac1 and M.A. Driancourt3

1 Reproductive Biology Unit, Dept of Obs–Gyn & Human Reproduction, CHU Tours, Faculté de Médecine, 37 044 Tours cedex, 2 INRA Xenobiotics, Toulouse and 3 INRA–URA CNRS1291, PRMD, Nouzilly, France

The aim of this study was to assess whether human dominant follicular fluid has the ability to modulate aromatase activity and/or granulosa cell proliferation. Dominant follicular fluid was obtained by laparoscopy before the luteinizing hormone surge in naturally cycling women while granulosa cells used in the tests were obtained from in-vitro fertilization patients. Aromatase was measured by the tritiated water release assay, following a 48 h incubation with follicular fluid and serum, and expressed for 5x104 granulosa cells. The effects of a range of follicular fluid or serum concentrations (2.5, 5, 10 and 20%) were compared. A decrease in aromatase activity was observed when high follicular fluid concentrations (20%) (P < 0.01) were added. Low concentrations (2.5%) of follicular fluid significantly increased cell proliferation (P < 0.01) as compared to basal values (0%). No further stimulation was however observed when concentrations increased up to 20%. Further characterization of these compounds is required to understand how they may modulate maturation of the dominant follicle.

Key words: aromatase/follicular fluid/gonadotrophins/granulosa cells/human

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