Human Reproduction, Vol. 14, No. 5, 1318-1323,
May 1999
© 1999 European Society of Human Reproduction and Embryology
The probability of abnormal preimplantation development can be predicted by a single static observation on pronuclear stage morphology
1 Laboratoire d'Eylau, 55 Rue Saint-Didier, 75116 Paris, France and 2 Centre of Reproductive Medicine, European Hospital,Via Portuense 700, 00149 Rome, Italy
This retrospective study was undertaken to determine whether further developmental progression of twopronucleated (2PN) zygotes can be predicted by a single, non-invasive examination of pronuclei, with the use of criteria based on the number and distribution of nucleolar precursor bodies in each pronucleus. The normal range of pronuclear variability was defined by analysis of zygotes giving rise to embryos transferred in 100%-implantation cycles (pattern 0). Morphological patterns differing from pattern 0 were classified as patterns 1–5. The frequency of developmental arrest of pattern 0 zygotes was only 8.5% as compared with 31.6, 21.9, 30.0, 20.5 and 24.1% for patterns 1–5 respectively. Relationships of pronuclear patterns with blastomere multinucleation and cleaving embryo morphology were also noted. Clinical pregnancy was achieved in 22 of 44 (50%) treatment cycles in which at least one pattern 0 embryo was transferred, but only in two of 23 (9%) cycles in which only pattern 1–5 embryos were transferred. These data present new evaluation criteria which can be used to predict the developmental fate of human embryos as early as the pronuclear stage, without requiring repeated observations or an exact timing of pronuclear zygote inspection. Further prospective study is needed for clinical validation of these criteria.
Key words: nucleolar precursor bodies/preimplantation development/pronuclear development/zygote selection
3 To whom correspondence should be addressed
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