Human Reproduction, Vol. 14, No. 8, 2080-2088,
August 1999
© 1999 European Society of Human Reproduction and Embryology
Lack of correlation between vascular endothelial growth factor production and endothelial cell proliferation in the human endometrium
Monash University Department of Obstetrics and Gynecology, Monash Medical Centre, 246 Clayton Road, Clayton, Victoria, 3168 Australia 1 Present address: School of Life Science and Technology, Victoria University of Technology, McKechnie Street, St Albans, Victoria 3021, Australia
The role of vascular endothelial growth factor (VEGF) in endometrial angiogenesis was examined by measuring its production in human endometrial tissues from different stages of the menstrual cycle and relating these data to endothelial cell proliferation in the same tissues. Conditioned medium was collected from explant, and separated glandular epithelial and stromal cells cultured from 24 normal human endometrial biopsies and VEGF measured by enzyme-linked immunosorbent assay (ELISA). Immunohistochemistry was also used to assess VEGF and the percentage of proliferating microvessels in the samples. Wide variation in results between individual endometrial samples at each stage of the menstrual cycle was observed for all parameters measured. There was no significant difference in VEGF secretion by explant, glandular epithelial or stromal cell cultures across the menstrual cycle, or in the percentage of proliferating vessels. VEGF immunostaining in the stroma was elevated during the early proliferative stage (P = 0.03). Epithelial cells secreted more VEGF than stromal cells (1.76 ± 0.46 versus 0.46 ± 0.06 ng per 105 cells; P = 0.002). There was no correlation between VEGF secreted by cultured explants, epithelial or stromal cells, VEGF immunostaining and the proportion of proliferating microvessels. These results show that the majority of endometrial VEGF is produced by glands, but neither total glandular nor stromal VEGF is correlated with endometrial endothelial cell proliferation. There is still no clear understanding on the regulation of human endometrial angiogenesis.
Key words: angiogenesis/endometrium/endothelial cell/human/VEGF
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