Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Burguete, T.
Right arrow Articles by Schlehofer, J. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Burguete, T.
Right arrow Articles by Schlehofer, J. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 14, No. 9, 2396-2401, September 1999
© 1999 European Society of Human Reproduction and Embryology

Evidence for infection of the human embryo with adeno-associated virus in pregnancy

Tatiana Burguete1, Michèle Rabreau2, Marianne Fontanges-Darriet3, Edith Roset4, Hans-Dieter Hager5, Alexandra Köppel5, Paul Bischof4 and Jörg R. Schlehofer1,6

1 Deutsches Krebsforschungszentrum (DKFZ), Angewandte Tumorvirologie, Im Neuenheimer Feld 242, D-69120 Heidelberg, Germany, 2 Institut d'Histo Cyto Pathologie, 114 Av Léon Blum,F-33194 Le Bouscat, France, 3 Cabinet de Chirurgie Gynécologique et d'Obstétrique, Echographie et Diagnostic Ante-Natal, Polyclinique Jean Villar, Av Maryse-Bastié, F-33520 Bruges/Bordeaux, France, 4 Département de Gynecologie et Obstétrique, Hôpital Contonal Universitaire, 32bis, Bd. de la Cluse, CH-1211 Genève 14, Switzerland and 5 Institut für Humangenetik, Abteilung Zytogenetik, Universität Heidelberg, Im Neuenheimer Feld 328,D-69120 Heidelberg, Germany

Previous reports have demonstrated the presence of DNA of the human helper virus-dependent adeno-associated parvovirus (AAV) in uterine tissue and curettage material from early miscarriage. To examine infection of embryonic tissue during pregnancy, amnion fluids were analysed for the presence of AAV. Using polymerase chain reaction, AAV DNA was detected in 64 out of 238 DNA samples extracted from amnion cells. DNA of helper viruses were found in 12% (papillomavirus) and 18% (cytomegalovirus) of the samples (double infections with AAV in eight and nine cases, respectively). Furthermore, infectious AAV virions were found in 13 out of 43 AAV DNA-containing samples. In mothers with AAV DNA-positive amnion fluids, premature amniorrhexis and premature labour occurred significantly more frequently (P < 0.001). Using an immunofluorescence assay, 24% of newborn sera (unrelated to the amnion fluid samples) were found to contain IgM antibodies to AAV, in most cases paralleled by IgM antibodies in the mother's sera. The data demonstrate that AAV infection can occur in utero at early and at late stages of pregnancy. The observed complications at delivery should encourage studies to clarify possible pathological consequences of AAV infection in pregnancy and a possible latent infection of the fetus.

Key words: adeno-associated virus/amniocentesis/in-utero infection/trophoblast cells/virus infection in pregnancy

6 To whom correspondence should be addressed


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
F. Arechavaleta-Velasco, L. Gomez, Y. Ma, J. Zhao, C.M. McGrath, M.D. Sammel, D.B. Nelson, and S. Parry
Adverse reproductive outcomes in urban women with adeno-associated virus-2 infections in early pregnancy
Hum. Reprod., January 1, 2008; 23(1): 29 - 36.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
F. Arechavaleta-Velasco, Y. Ma, J. Zhang, C. M. McGrath, and S. Parry
Adeno-Associated Virus-2 (AAV-2) Causes Trophoblast Dysfunction, and Placental AAV-2 Infection Is Associated with Preeclampsia
Am. J. Pathol., June 1, 2006; 168(6): 1951 - 1959.
[Abstract] [Full Text] [PDF]

Home page
 Mol Cancer ResHome page
H. You, Y. Liu, M. J. Carey, C. L. Lowery, and P. L. Hermonat
Defective 3A Trophoblast-Endometrial Cell Adhesion and Altered 3A Growth and Survival by Human Papillomavirus Type 16 Oncogenes
Mol. Cancer Res., November 1, 2002; 1(1): 25 - 31.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
K. Erles, V. Rohde, M. Thaele, S. Roth, L. Edler, and J. R. Schlehofer
DNA of adeno-associated virus (AAV) in testicular tissue and in abnormal semen samples
Hum. Reprod., November 1, 2001; 16(11): 2333 - 2337.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.