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Human Reproduction, Vol. 14, No. suppl_1, pp. 186-193, 1999
© 1999 European Society of Human Reproduction and Embryology

Possible applications of lasers in assisted reproductive technologies

B. Schöpper1, M. Ludwig, J. Edenfeld, S. Al-Hasani and K. Diedrich

Department of Obstetrics and Gynecology, Medical University of Lübeck Ratzeburger Allee 160, 23538 Lübeck, Germany

Correspondence: 1To whom correspondence should be addressed

Laser systems seem to be the most promising technical tools to be introduced in assisted reproduction treatment in recent years. The 1.48 µm diode laser in particular is on course to supersede conventional mechanical and chemical methods for opening the zona pellucida in assisted hatching, and for biopsy of polar bodies or blastomeres. This is because it works without physically touching the cells, has no traceable toxic effects on living cells and is easy to handle. There will be manifold future possibilities for micromanipulation with lasers, as already demonstrated by the immobilization of spermatozoa prior to intracytoplasmic sperm injection, and the successful dissection of the zona pellucida into two halves with equal diameter for the hemizona assay.

Key words: ART/embryo biopsy/hemizona assay/laser/preimplantation genetic diagnosis


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