Skip Navigation

This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Veiga, A.
Right arrow Articles by Barri, P.N.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Veiga, A.
Right arrow Articles by Barri, P.N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 14, No. suppl_2, pp. 112-120, 1999
© 1999 European Society of Human Reproduction and Embryology

Use of co-culture of human embryos on Vero cells to improve clinical implantation rate

A. Veiga1,2,3, M.J. Torelló1, Y. Ménézo2, A. Busquets1, O. Sarrias1, B. Coroleu1 and P.N. Barri1

1 Reproductive Medicine Service, Department of Obstetrics and Gynecology, Institut Universitari Dexeus Barcelona, Spain 2 Institut Rhônalpin, Fondation Marcel Merieux Bron, France

Correspondence: 3To whom correspondence should be addressed

Co-culture of human embryos (n = 384 cycles) to the blastocyst stage using Vero cell monolayers was carried out between August 1995 and December 1997. A total of 2868 zygotes were co-cultured and 1027 embryos reached the blastocyst stage (blastocyst formation rate 35.8%). The blastocysts were frozen in 43.7% of patients. A mean of 1.8 blastocysts was transferred per patient and 95 pregnancies were obtained (pregnancy rate/cycle 24.7%). The blastocyst implantation rate was 23.6%. Miscarriage occurred in 15 patients (15.7%) and ectopic pregnancy in three (3.1%) patients. The multiple pregnancy rate was 32.6%. No differences were observed in the blastocyst rate between poor, normal or high response patients. Blastocyst formation was significantly lower when frozen donor spermatozoa were used. Significantly higher pregnancy rates per transfer and blastocyst implantation rates were attained when embryos were transferred on days 5 or 6 compared with day 7. No advantage was observed when co-culture was used in first cycle IVF patients, in comparison with conventional day 2 replacements. The use of blastocysts for preimplantation genetic diagnosis (PGD) increases the diagnostic reliability and widens diagnostic possibilities. A total of 215 cycles with frozen–thawed co-cultured blastocysts were carried out, with a pregnancy rate of 22.7% per replacement.

Key words: blastocyst transfer/co-culture/frozen–thawed blastocyst transfer/implantation rates/preimplantation genetic diagnosis


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
S. Friedler, M. Schachter, D. Strassburger, K. Esther, R. Ron El, and A. Raziel
A randomized clinical trial comparing recombinant hyaluronan/recombinant albumin versus human tubal fluid for cleavage stage embryo transfer in patients with multiple IVF-embryo transfer failure
Hum. Reprod., September 1, 2007; 22(9): 2444 - 2448.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.