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Human Reproduction, Vol. 15, No. 1, 50-54, January 2000
© 2000 European Society of Human Reproduction and Embryology

Elevation of insulin-like growth factor-binding protein-1 mRNA expression following hormone replacement therapy

Hsin-Shih Wang1, Tzu-Hao Wang and Yung-Kuei Soong

Department of Obstetrics & Gynecology, Chang-Gung Medical College, Chang-Gung Memorial Hospital, Lin-Kou Medical Center, Taipei, Taiwan, Republic of China

The purpose of this study was to investigate the possible regulatory roles of insulin-like growth factors (IGF) and IGF binding protein-1 (IGFBP-1) on postmenopausal endometrium in women undergoing hormone replacement therapy (HRT). Endometrial tissues and blood samples were collected from 25 postmenopausal women with abnormal uterine bleeding before and after HRT. As a control, blood and endometrial samples were also obtained at mid-luteal phase (days 18–23 of the menstrual cycle) from 10 women with benign uterine leiomyoma during surgical intervention. Expression of mRNA for IGF-I, IGF-II, type 1-IGF receptor, IGFBP-1, oestrogen receptor (ER) and progesterone receptor (PR) in the endometrium was explored by a semiquantitative reverse transcription–polymerase chain reaction (RT-PCR). Serum concentrations of IGFBP-1, oestradiol, progesterone, follicle stimulating hormone (FSH) and sex hormone-binding globulin (SHBG) were also determined. In the endometrium obtained from postmenopausal women with abnormal uterine bleeding (before HRT), expression of IGFBP-1 was undetectable, whereas PR expression was abundant. HRT significantly up-regulated expression of IGFBP-1 but down-regulated PR. Moderate expression of IGF-I, IGF-II, type 1-IGF receptor and ER was identified in the postmenopausal endometrium before HRT. HRT significantly down-regulated IGF-I, up-regulated IGF-II, but had no effects on expression of type 1-IGF receptor and ER. In conclusion, up-regulation of IGFBP-1 collaboratively with down-regulation of IGF-I may account for the protective effect of progesterone in HRT on the endometrium.

Key words: endometrium/hormone replacement therapy/IGF/IGFBP-1/progesterone

1 To whom correspondence should be addressed


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 J. Clin. Endocrinol. Metab.Home page
T.-H. Wang, S.-G. Horng, C.-L. Chang, H.-M. Wu, Y.-J. Tsai, H.-S. Wang, and Y.-K. Soong
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