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Human Reproduction, Vol. 15, No. 10, 2186-2189, October 2000
© 2000 European Society of Human Reproduction and Embryology

Safety of cryopreservation straws for human gametes or embryos: a preliminary study with human immunodeficiency virus-1

Jean-Louis Benifla1,6, Helene Letur-Konïrsch2,3, Gille Collin4, Aviva Devaux5,5, Frederique Kuttenn2, Patrick Madelenat1, Françoise Brun-Vezinet4 and Gerard Feldmann5

1 Department of Obstetrics and Gynaecology, Hôpital Bichat, 75018 Paris, 2 Cecos, Hôpital Necker, 75007 Paris, 3 Centre de Fertilité, Institut Mutualiste Montsouris, 75014 Paris, 4 Department of Virology, Hôpital Bichat, 75018 Paris and 5 Department of Histo-embryology, Hôpital Bichat, 75018 Paris, France

The aim of this preliminary experimental study was to test the stability of cryopreservation straws to human immunodeficiency virus-1 (HIV-1). Three kinds of straws were tested: four polyvinyl chloride (PVC), four polyethylene terephthalate glycol (PETG) and 20 high-security ionomeric resin (IR). The PVC and PETG straws were sealed ultrasonically, and the IR straw by thermosoldering. Each sealed straw was cut in half to produce two demi-straws and then filled with 100 µl of HIV-1-containing supernatant (reverse transcriptase activity: 15 000 c.p.m./50 µl). The unsealed cotton end of PVC and PETG straws and the two halves of the IR straws (cotton and plastic plug ends) were tested. Each demi-straw was two- thirds submerged in RPMI medium at 37°C, and RPMI samples were withdrawn on days 3, 7 and 11. Viral RNA was extracted from the medium and then amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) followed by nested PCR using primers specific to HIV-1 protease. On day 7, no HIV-1 RNA was detected in any of the different samples of medium that had surrounded the unsealed PVC and PETG straws with cotton ends, but three IR specimens were positive. On day 11, PVC and PETG remained negative but HIV-1 RNA was detected in RPMI samples for two more IR demi-straws (n = 5). In conclusion, under these experimental conditions (at 37°C), the unsealed cotton end PVC, PETG and thermosoldered cotton end IR demi-straws appeared to be safe for HIV-1, while IR straws, sealed or unsealed with a plastic plug and with unsealed cotton ends, leaked.

Key words: assisted reproductive technology/cryopreservation/experimental study/HIV/straw

6 To whom correspondence should be addressed at: Service de Gynécologie-Obstétrique, Hôpital Bichat, 170 bd Ney, 75877 Paris Cedex 18, France. E-mail: jean-louis.benifla{at}bch.ap-hop-paris.fr


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