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Human Reproduction, Vol. 15, No. 12, 2531-2535, December 2000
© 2000 European Society of Human Reproduction and Embryology

Microsurgical epididymal sperm aspiration: aspirate analysis and straws available after cryopreservation in patients with non-reconstructable obstructive azoospermia

I. Schroeder-Printzen1, J. Zumbé2, L. Bispink3, St. Palm4, U. Schneider5,5, U. Engelmann2, W. Weidner1 and The MESA/TESE Group Giessen*

1 Department of Urology, University of Giessen and 2 Department of Urology, University of Cologne and 3 Outpatient Department of Obstetrics and Gynaecology, Bad Münder and 4 Outpatient Department for Reproductive Medicine, Cologne and 5 Clinic for Reproductive Medicine GmbH, Bad Münder, Germany

Microsurgical epididymal sperm aspiration (MESA) combined with intracytoplasmic sperm injection (ICSI) represents a great advance in the therapy of non-reconstructable obstructive azoospermia. For procedure synchronization, a great number of organizational facilities are needed. Intentional cryopreservation of the aspirate may reduce these problems, therefore the aim of this study was to analyse the amount and quality of aspirate fluid obtained by means of MESA and the quality of the vials after thawing. Furthermore, the available cryopreserved straws were calculated. A total of 93 consecutive MESA procedures were performed and epididymal spermatozoa were obtained in 88 patients. Mean sperm concentration was 40.9x106 spermatozoa/ml. Global and progressive motility were 24.8 and 7.5% respectively. In one-third of the aspirates, no progressive motile spermatozoa were found. The mean number of straws available was 7.6. In 33 ICSI cycles with frozen–thawed epididymal spermatozoa, a pregnancy rate of 42.4% was achieved. In conclusion, these data show that enough spermatozoa are available for various ICSI cycles following a single MESA procedure in men with non-reconstructable obstructive azoospermia. Furthermore, ICSI with cryopreserved spermatozoa leads to excellent pregnancy rates

Key words: assisted reproductive techniques/infertility/microsurgical epididymal sperm aspiration/obstructive azoospermia

5 To whom correspondence should be addressed at: Department of Urology, Justus-Liebig University, Klinikstrasse 29, 35385 Giessen, Germany. E-mail: Immo.Schroeder-Printzen{at}chiru.med.uni-giessen.de

* Members of the MESA/TESE GROUP GIESSEN in alphabetical order: H.J.G.Herrero, F.Köhn, W.Künzel, W.-B.Schill, I.Schroeder-Printzen, R.Stalf, W.Weidner.


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R. Lee, P.S. Li, M. Goldstein, C. Tanrikut, G. Schattman, and P.N. Schlegel
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[Abstract] [Full Text] [PDF]


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