Human Reproduction, Vol. 15, No. 12, 2610-2620,
December 2000
© 2000 European Society of Human Reproduction and Embryology
ICSI choreography: fate of sperm structures after monospermic rhesus ICSI and first cell cycle implications
1 Oregon Regional Primate Research Center, Division of Reproductive Sciences, Beaverton, Oregon, 2 Departments of Obstetrics and Gynecology, and Cell & Developmental Biology, and Center for Women's Health, Oregon Health Sciences University, Portland, Oregon, USA 3 Department of Anatomy and Cell Biology, Queens University, Kingston, Ontario, Canada, 4 Department of Molecular and Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA and 5 Center for Neuroscience and Cell Biology of Coimbra, Department of Zoology, University of Coimbra, 3000 Coimbra, Portugal
We have dissected the initial stages of fertilization by intracytoplasmic sperm injection of single spermatozoa into prime oocytes from fertile rhesus monkeys (Macaca mullata). DNA decondensation was delayed at the apical portion of the sperm head. It is possible that this asynchronous male DNA decondensation could be related to the persistence of the sperm acrosome and perinuclear theca after injection. However, incomplete male pronuclear formation did not prevent sperm aster formation, microtubule nucleation and pronuclear apposition. In contrast, DNA synthesis was delayed in both pronuclei until the sperm chromatin fully decondensed, indicating that male pronuclear formation constitutes an important checkpoint during the first embryonic cell cycle.
Key words: acrosome/fertilization/ICSI/perinuclear theca/spermatozoa
6 Current address: Center for Neuroscience and Cell Biology of Coimbra, Department of Zoology, University of Coimbra, 3000 Coimbra, Portugal
7 To whom correspondence should be addressed at: Oregon Regional Primate Research Center, Oregon Health Sciences University, 505 NW 185th Avenue, Beaverton, OR 97006, USA. E-mail: schatten{at}ohsu.edu
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