Human Reproduction, Vol. 15, No. 2, 264-272,
February 2000
© 2000 European Society of Human Reproduction and Embryology
Evidence for the involvement of blood flow-related mechanisms in the ovulatory process of the rat
1 Department of Obstetrics and Gynecology and 2 Department of Surgery, Göteborg University, Sweden
To elucidate whether any relationship exists between ovarian blood flow and ovulation rate, the effects on these parameters were examined in equine chorionic gonadotrophin/human chorionic gonadotrophin (eCG/HCG) (15I U/15I U) primed rats after bilateral ligation and severance of either the ovarian branch of the uterine artery and vein (UL), the ovarian artery and vein (OL) or both sites (UL+OL) in comparison to sham operations. Laser Doppler flowmetry demonstrated the presence of microcirculatory vasomotion and a reduction of blood flow after UL, OL and UL+OL performed during the intervals 0–3 h (78, 66 and 19% of pretreatment values respectively) and 6–9 h (68, 57 and 20%) after HCG. Experiments utilizing radioactive microspheres also indicated decreased ovarian blood flow by UL and OL. Ovulation rate was assessed 20 h after HCG in animals where ligations had been performed at 0, 3, 6 and 9 h after HCG. No ovulations were seen after UL+OL and significantly decreased ovulation rates (~50% of sham operated animals) were seen after UL at 0 and 3 h and after OL at 0, 6 and 9 h. Progesterone concentrations in blood 20 h after HCG were reduced by OL but not UL and ovarian weights were unaffected by ligation. It is concluded that acute blood flow reduction during the ovulatory interval reduces ovulation rate in the rat.
Key words: blood flow/laser Doppler/ovary/ovulation/rat
3 Present address: Department of Obstetrics and Gynecology, University of Utah, 50 North Medical Drive, Salt Lake City, Utah 84132, USA
4 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, Göteborg University, Sahlgrenska University Hospital, S-413 45 Göteborg, Sweden
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