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Human Reproduction, Vol. 15, No. 4, 857-860, April 2000
© 2000 European Society of Human Reproduction and Embryology

The use of silane-coated silica particles for density gradient centrifugation in in-vitro fertilization

Brita Söderlund1 and Kersti Lundin

Department of Obstetrics and Gynecology, University of Göteborg, SU/Sahlgrenska, SE 413 45 Göteborg, Sweden

Silane-coated silica particles (PureSperm®) were evaluated as an alternative to Percoll for gradient separation of spermatozoa, for use in assisted reproduction. Recovery of motile and morphologically normal spermatozoa after using a four-layer Percoll and a two- and four-layer PureSperm® gradient respectively was recorded. In-vitro fertilization (IVF) results after using PureSperm® for the sperm preparation were also evaluated. No difference in sperm recovery or sperm motility was found when comparing the use of Percoll and the four-layer gradient of PureSperm®. When using a two-layer PureSperm® gradient, motility was significantly decreased (P < 0.05) compared to Percoll. Normal sperm morphology increased from 8–17.2% after using Percoll and to 12.7% and 11.4% after using a four-layer and a two-layer PureSperm® gradient respectively. All gradient preparations showed a significant decrease in the teratozoospermia index compared to the ejaculate (P < 0.01). No significant differences in IVF results regarding fertilization and pregnancy rates were found when PureSperm® or the swim-up technique were used for the sperm preparation. PureSperm® seems to be an acceptable alternative to Percoll but although the percentage of sperm recovery was higher after PureSperm® we still recommend the swim-up technique to be the first choice, as a higher percentage of progressive motile spermatozoa is obtained without using other chemicals than IVF culture medium.

Key words: IVF/Percoll/PureSperm®/sperm morphology/swim-up

1 To whom correspondence should be addressed


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