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Human Reproduction, Vol. 15, No. 5, 1200-1204, May 2000
© 2000 European Society of Human Reproduction and Embryology

Equal distribution of congenital blood cell chimerism in dizygotic triplets after in-vitro fertilization: Case report

Rita Kühl-Burmeister1,6, Eva Simeoni2, Klaus Weber-Matthiesen3, Antje Milde2, Catherine Herwartz4, Jürgen Neppert1 and Meinolf Suttorp5

1 Department of Transfusion Medicine, 2 Forensic Medicine, 3 Human Genetics, 4 Immunology and 5 Paediatrics, University of Kiel Medical School, Michaelisstrasse 5, D-24105 Kiel, Germany

The special situation of multiple pregnancies following IVF has led to a growing interest in the assessment of embryonal development by means of molecular genetics. We report a case of congenital blood chimerism in dizygotic triplets (two boys, one girl) present in erythrocytes and leukocytes in both sexes. Routine pre-operative blood serology of the 6 year old female triplet revealed chimerism of the red cells. Flow cytometry of the erythrocytes and DNA analysis of the leukocytes demonstrated that all three children had the same proportions of male and female cells. Fluorescent in-situ hybridization (FISH) analyses revealed Y chromosomes in 84% of the girl's leukocytes and in 89/92% of the two boys' leukocytes. The true genetic lines were determined by analysing polymorphism of serum groups (glycoprotein, transferrin, protease inhibitor and plasminogen) secreted by non-haematopoetic tissue, by blood group typing of hair roots and by DNA analysis of endothelial cells. Evidently placental anastomoses allowed a reciprocal intra-uterine transfusion of blood stem cells in the triplets.

Key words: blood chimerism/dizygotic triplets/in-vitro fertiliz-ation

6 To whom correspondence should be addressed


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