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Human Reproduction, Vol. 15, No. 7, 1597-1603, July 2000
© 2000 European Society of Human Reproduction and Embryology

Establishment of a ciliated epithelial cell line from human Fallopian tube

Hisao Ando1,2,4, Miya Kobayashi3, Shigeru Toda1, Fumitaka Kikkawa1,2, Tetsuo Masahashi1 and Shigehiko Mizutani1,2

1 Department of Obstetrics and Gynecology, Nagoya University School of Medicine, 2 Maternity and Perinatal Care Center, Nagoya University Hospital and 3 Department of Anatomy, Nagoya University School of Medicine, Nagoya, Japan

Human tubal epithelial cells in primary culture were transfected with simian virus 40 (SV40) large T antigen plasmid, and an immortalized ciliated cell line, named as NT/T-S, was established without crisis. Transmission electron microscopy proved that NT/T-S cells had cilia, microvilli, junctional complexes, rough endoplasmic reticula, free ribosomes and microtubules. NT/T-S cells were evaluated preliminarily on the basis of co-culture study using surplus embryos at the 4- to 8-cell stage in our IVF and embryo transfer programme. All of the 133 embryos had >=10% fragments (based on the surface area) and were unworthy of cryopreservation. Up to 57% (16/28) of the embryos with 10–30% fragments reached the blastocyst stage by co-culture. In contrast, blastocyst formation was observed in <10% of the control embryos, some of which were co-cultured with NFL/T cells (the immortalized human fetal liver epithelial cells) (1/16), and the others were incubated with the co-culture medium alone (1/18). Various cytokines/growth factors such as leukaemia inhibitory factor (LIF), interleukin (IL)-6, IL-8 and basic fibroblast growth factor were secreted by NT/T-S cells as well as by the tubal epithelial cells in primary culture. The establishment of a ciliated cell line will provide a valuable resource for the further studies of the Fallopian tube in the early events of pregnancy.

Key words: co-culture/human blastocyst/in-vitro fertilization/leukaemia inhibitory factor/oviduct

4 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, Nagoya University School of Medicine, Nagoya 466-8550, Japan. E-mail: ando{at}tsuru.med.nagoya-u.ac.jp


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