Human Reproduction, Vol. 15, No. 8, 1685-1690,
August 2000
© 2000 European Society of Human Reproduction and Embryology
Impact of oestradiol and inhibin A concentrations on pregnancy rate in in-vitro oocyte maturation
The Fertility Clinic, Herlev University Hospital, Denmark
The purpose of the present study was to analyse the results of maturation of oocytes obtained in unstimulated normal women after a leading follicle of 10 mm diameter and an endometrium of at least 5 mm thickness were observed at ultrasound. The serum concentrations of oestradiol and inhibin A were analysed from day 3 to the day of aspiration and retrospectively evaluated. A total of 75 normal regular cycling women referred for IVF/intracytoplasmic sperm injection (ICSI) because of male factor and/or tubal disease was included (n = 87 consecutive cycles). The oocytes were aspirated transvaginally and matured for 28–36 h. ICSI was performed on all metaphase II oocytes and they were cultured to day 2 or 3 after insemination, at which time suitable embryos (maximum two) were replaced into the women. Eleven singleton pregnancies with a live fetus were obtained after transfer in 63 cycles (pregnancy rate of 13% per aspiration and 17% per transfer). Nine healthy children have been born and the remaining two pregnancies miscarried in the eighth to ninth gestational week. The serum concentrations of oestradiol on day 3 and on the day of aspiration were available in 85 cycles, and in 57 of these an increase of 100% was detected on the day of aspiration. Significantly more pregnancies were observed in these cycles compared with cycles without an increase in the concentration of oestradiol (19 versus 0% per aspiration, 24 versus 0% per transfer, P < 0.02). Further improvement in pregnancy rate was obtained if aspiration was performed after increase in inhibin A concentration (24 versus 0% per aspiration and 33 versus 0% per transfer, P < 0.02) (n = 83 cycles, where inhibin concentrations were available, and n = 42 cycles, where an increase of 80% was detected on the day of aspiration compared with day 3). Timing of aspiration may improve the developmental potential of immature oocytes.
Key words: human oocytes/maturation in vitro/pregnancy rate/timing of aspiration
1 To whom correspondence should be addressed at: The Fertility Clinic, Herlev University Hospital, DK-2730 Herlev, Denmark.E-mail: alm{at}ciconia.dk
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