Human Reproduction, Vol. 16, No. 10, 2056-2060,
October 2001
© 2001 European Society of Human Reproduction and Embryology
Ovarian tissue harvested from lymphoma patients to preserve fertility may be safe for autotransplantation
1 Centre for Reproduction, Growth and Development, University of Leeds, Leeds, 2 Department of Medical Oncology, 3 Department of Histopathology, Christie Hospital NHS Trust, Manchester, 4 Department of Cancer Genetics, Paterson Institute for Cancer Research, Manchester, 5 Department of Reproductive Medicine, St. Mary's Hospital, Manchester and 6 Department of Endocrinology, Christie Hospital NHS Trust, Manchester, UK
BACKGROUND: Ovarian failure is a common sequel to chemo/radiotherapy in patients successfully treated for cancer. Harvesting, cryopreserving and subsequently re-implanting ovarian cortical grafts can be used to re-establish reproductive potential in women with cancer. The safety issue, however, is of great concern because residual disease in autografted ovarian tissues might cause recrudescence of disease. METHODS: A total of 30 non-obese diabetic severe combined immunodeficient (NOD/LtSz-SCID) mice were individually xenografted s.c. with frozen–thawed ovarian tissue from 18 patients with lymphoma [13 Hodgkin's lymphoma (HL) and 5 non-Hodgkin's lymphoma (NHL)]. The animals were autopsied at 16 weeks, or earlier if cachectic. The xenograft, liver, spleen, sternum, para-aortic lymph nodes and thymus were prepared for histology, immunohistochemistry and human DNA microsatellite analysis. RESULTS: None of the animals grafted with ovarian tissue from lymphoma patients developed disease. However, all 3 animals grafted with lymph node tissue from an NHL patient developed B-cell lymphomas that were confirmed as human in origin by DNA microsatellite analysis. CONCLUSION: Ovarian tissue harvested before high-dose chemotherapy for HL or NHL may not carry a risk of disease transmission by autotransplantation, although the possibility is difficult to exclude completely.
Key words: cryopreservation/fertility/lymphoma/transplantation/xenografting
7 Present address: Women's Pavilion, Royal Victoria Hospital, 687 Pine Avenue West, Montreal H3A 1A1, Quebec, Canada
8 To whom correspondence should be addressed at: University of Washington, Department of Obstetrics and Gynecology,4225 Roosevelt Way N.E., Suite 305, Seattle WA 98105, USA. E-mail: medssk{at}att.net
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