Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Ebner, T.
Right arrow Articles by Tews, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ebner, T.
Right arrow Articles by Tews, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 16, No. 12, 2628-2631, December 2001
© 2001 European Society of Human Reproduction and Embryology

Laser assisted immobilization of spermatozoa prior to intracytoplasmic sperm injection in humans

T. Ebner,1, C. Yaman, M. Moser, M. Sommergruber, J. Hartl and G. Tews

Women's General Hospital, IVF-Unit, A-4020 Linz, Austria

BACKGROUND: The conventional method of immobilization of spermatozoa prior to intracytoplasmic sperm injection (ICSI) is mechanical breakage of the tail by pressing it against the bottom of the injection dish. METHODS: This prospective self-controlled study was set up to evaluate the potential of a non-contact 1.48 µm wavelength diode laser in terms of immobilization. In addition, the fertilization rate and further development potential of such zygotes were investigated. The patients included in our study (n = 60) had oestradiol concentrations >2000 pg/ml, and thus a relatively high number of MII oocytes could be expected. Approximately half the oocytes were injected with laser treated spermatozoa (n = 262, study group) and the other half with mechanically immobilized spermatozoa (n = 252, control group). RESULTS: No significant differences between the two groups in terms of fertilization rate, early cleavage or blastocyst formation were observed. However, time required for identification, aspiration and injection of a potential spermatozoa was significantly shorter in the laser immobilized sperm group (P < 0.001). CONCLUSIONS: The application of a non-contact diode laser for sperm immobilization prior to ICSI is a potentially useful alternative to the conventional mechanical approach.

Key words: blastocyst formation/early cleavage/fertilization rate/immobilization of spermatozoa/non-contact laser

1 To whom correspondence should be addressed. E-mail: Thomas.Ebner{at}li.lkh.ooe.gv.at


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
T. Ebner, M. Moser, and G. Tews
Possible applications of a non-contact 1.48 {micro}m wavelength diode laser in assisted reproduction technologies
Hum. Reprod. Update, July 1, 2005; 11(4): 425 - 435.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
T. Takeuchi, L. T. Colombero, Q. V. Neri, Z. Rosenwaks, and G. D. Palermo
Does ICSI require acrosomal disruption? An ultrastructural study
Hum. Reprod., January 1, 2004; 19(1): 114 - 117.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
B. Balaban, K. Lundin, J.M. Morrell, H. Tjellstrom, B. Urman, and P.V. Holmes
An alternative to PVP for slowing sperm prior to ICSI
Hum. Reprod., September 1, 2003; 18(9): 1887 - 1889.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.