Human Reproduction, Vol. 16, No. 2, 300-305,
February 2001
© 2001 European Society of Human Reproduction and Embryology
Maturation of rhesus monkey oocytes in chemically defined culture media and their functional assessment by IVF and embryo development
1 Department of Reproductive Biology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 Jiao Chang Dong Lu, Kunming, Yunnan 650223, China, 2 Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI 53706, USA and 3 China-US Primate Biology Laboratory, Kunming Institute of Zoology, The Chinese Academy of Sciences, Kunming 650223, China
This study compared success of in-vitro maturation of rhesus monkey oocytes in protein-free versus serum-containing culture systems, assessed by embryo development subsequent to IVF. Four media were tested: (i) modified Connaught Medical Research Laboratories medium (mCMRL-1066); (ii) hamster embryo culture medium-10 (HECM-10); (iii) control: mCMRL-1066 + 20% bovine calf serum (BCS); (iv) HECM-10 + 20% BCS. Immature oocytes from FSH-stimulated rhesus monkeys were allocated among the media containing ovine FSH (5 µg/ml) and LH (10 µg/ml) and cultured for 36–40 h. Metaphase II ova were inseminated and putative zygotes were cultured in mCMRL-1066 + 20% BCS until development arrested. Ova matured in all four media had similar (P > 0.05) potential to initiate (66, 67, 82 and 69% respectively) and complete meiotic maturation (60, 50, 76 and 57% respectively). Inseminated ova in all groups had similar potential to be fertilized (86, 83, 84 and 90% respectively), cleave (71, 83, 76 and 90% respectively) and develop to the blastocyst stage (19, 17, 16 and 30% respectively). These results indicate for the first time that primate oocytes can be successfully matured in protein-free medium, with subsequent blastocyst development, comparable to responses in complex medium with serum. This finding will facilitate studies on mechanisms regulating primate oocyte maturation.
Key words: blastocysts/in-vitro maturation/primate oocytes/protein-free medium
4 Present address: Audubon Institute Center for Research of Endangered Species, 14001 River Road, New Orleans, LA 70131, USA
5 To whom correspondence should be addressed at: Department of Reproductive Biology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 Jiao Chang Dong Lu, Kunming, Yunnan 650223, China. E-mail: wji{at}mail.kiz.ac.cn
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. K. Nyholt de Prada, Y. S. Lee, K. E. Latham, C. L. Chaffin, and C. A. VandeVoort Role for cumulus cell-produced EGF-like ligands during primate oocyte maturation in vitro Am J Physiol Endocrinol Metab, May 1, 2009; 296(5): E1049 - E1058. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Zheng, W. Si, B. D. Bavister, J. Yang, C. Ding, and W. Ji 17{beta}-Estradiol and progesterone improve in-vitro cytoplasmic maturation of oocytes from unstimulated prepubertal and adult rhesus monkeys Hum. Reprod., October 1, 2003; 18(10): 2137 - 2144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. T. Davenport, C. J. Lees, H. L. Green, and K. A. Grant Long-Acting Depot Formulation of Luprolide Acetate as a Method of Hypothalamic Down Regulation for Controlled Ovarian Hyperstimulation and Oocyte Production in Macaca fascicularis Biol Reprod, June 1, 2003; 68(6): 2261 - 2266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.T. Jensen, K.M. Schwinof, M.B. Zelinski-Wooten, M. Conti, L.V. DePaolo, and R.L. Stouffer Phosphodiesterase 3 inhibitors selectively block the spontaneous resumption of meiosis by macaque oocytes in vitro Hum. Reprod., August 1, 2002; 17(8): 2079 - 2084. [Abstract] [Full Text] [PDF]
|
|