Human Reproduction, Vol. 16, No. 2, 333-336,
February 2001
© 2001 European Society of Human Reproduction and Embryology
Monozygotic twins and transfer at the blastocyst stage after ICSI
Clínica e Centro de Pesquisa em Reproducião Humana Roger Abdelmassih, São Paulo, Brasil
The incidence of monozygotic twinning (MZT) is higher in pregnancies conceived after assisted reproduction than after natural conception. Alterations, produced by ovarian stimulation, in-vitro culture conditions and specifically alterations of zona pellucida are mentioned as possible causes of this phenomenon. A retrospective review was performed of the incidence of MZT in pregnancies generated in our centre during the period of January 1996 to December 1999. This variable was compared in 129 gestations that resulted from blastocyst transfer (occurring from September 1998 to August 1999) with 814 pregnancies produced by transfers of 4- to 8-cell embryos. Follicular development was induced with human menopausal gonadotrophin and urinary FSH during 1996 and 1997 and with recombinant FSH during 1998 and 1999. Blastocysts were cultured in sequential media using S1 or G1 up to 72 h and S2 or G2 to day 5. Five of the 129 pregnancies generated by blastocyst transfers were complicated by MZT gestation (3.9%). In comparison, only six of 814 pregnancies occurred from 4- to 8-cell transfers (0.7%), a difference that is statistically significant (P < 0.001 with Yates correction). The results confirm an increase of MZT in pregnancies from intracytoplasmic sperm injection as compared to the natural incidence. Moreover, the frequency of MZT was significantly higher when transfers were performed at the blastocyst stage, suggesting that extended in-vitro culture of embryos may be associated with alterations of the zona pellucida and the hatching process.
Key words: blastocyst/ICSI/IVF/monozygotic twinning
1 To whom correspondence should be addressed. E-mail: clinica{at}dglnet.com.br
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  W. Verpoest, L. Van Landuyt, S. Desmyttere, A. Cremers, P. Devroey, and I. Liebaers The incidence of monozygotic twinning following PGD is not increased Hum. Reprod., November 1, 2009; 24(11): 2945 - 2950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Vitthala, T.A. Gelbaya, D.R. Brison, C.T. Fitzgerald, and L.G. Nardo The risk of monozygotic twins after assisted reproductive technology: a systematic review and meta-analysis Hum. Reprod. Update, January 1, 2009; 15(1): 45 - 55. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K I Aston, C M Peterson, and D T Carrell Monozygotic twinning associated with assisted reproductive technologies: a review Reproduction, October 1, 2008; 136(4): 377 - 386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. C. Skiadas, S. A. Missmer, C. B. Benson, R. E. Gee, and C. Racowsky Risk factors associated with pregnancies containing a monochorionic pair following assisted reproductive technologies Hum. Reprod., June 1, 2008; 23(6): 1366 - 1371. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kanaya, Y. Murata, H. Oku, S. Hashimoto, Y. Morimoto, T. Murata, K. Sugihara, F. Nagata, Y. Nakaoka, and A. Fukuda Successful monozygotic twin delivery following in vitro maturation of oocytes retrieved from a woman with polycystic ovary syndrome: Case Report Hum. Reprod., July 1, 2006; 21(7): 1777 - 1780. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.M. Kolibianakis, K. Zikopoulos, W. Verpoest, M. Camus, H. Joris, A.C. Van Steirteghem, and P. Devroey Should we advise patients undergoing IVF to start a cycle leading to a day 3 or a day 5 transfer? Hum. Reprod., November 1, 2004; 19(11): 2550 - 2554. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-P. Primi, A. Senn, M. Montag, H. Van der Ven, J. Mandelbaum, A. Veiga, P. Barri, and M. Germond A European multicentre prospective randomized study to assess the use of assisted hatching with a diode laser and the benefit of an immunosuppressive/antibiotic treatment in different patient populations Hum. Reprod., October 1, 2004; 19(10): 2325 - 2333. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Wright, L. A. Schieve, A. Vahratian, and M. A. Reynolds Monozygotic twinning associated with day 5 embryo transfer in pregnancies conceived after IVF Hum. Reprod., August 1, 2004; 19(8): 1831 - 1836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Moser, T. Ebner, M. Sommergruber, U. Gaisswinkler, K. Jesacher, M. Puchner, R. Wiesinger, and G. Tews Laser-assisted zona pellucida thinning prior to routine ICSI Hum. Reprod., March 1, 2004; 19(3): 573 - 578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Zikopoulos, P. Platteau, E. Kolibianakis, C. Albano, A. Van Steirteghem, and P. Devroey Quintuplet pregnancy following transfer of two blastocysts: Case report Hum. Reprod., February 1, 2004; 19(2): 325 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Unger, M. Hoopmann, R. Bald, D. Foth, and F. Nawroth Monozygotic triplets and monozygotic twins after ICSI and transfer of two blastocysts: case report Hum. Reprod., January 1, 2004; 19(1): 110 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Moriwaki, N. Suganuma, M. Hayakawa, H. Hibi, Y. Katsumata, H. Oguchi, and M. Furuhashi Embryo evaluation by analysing blastomere nuclei Hum. Reprod., January 1, 2004; 19(1): 152 - 156. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Emiliani, A. Delbaere, A.-S. Vannin, J. Biramane, M. Verdoodt, Y. Englert, and F. Devreker Similar delivery rates in a selected group of patients, for day 2 and day 5 embryos both cultured in sequential medium: a randomized study Hum. Reprod., October 1, 2003; 18(10): 2145 - 2150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Alikani, N. A. Cekleniak, E. Walters, and J. Cohen Monozygotic twinning following assisted conception: an analysis of 81 consecutive cases Hum. Reprod., September 1, 2003; 18(9): 1937 - 1943. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Blickstein, C. Jones, and L. G. Keith Zygotic-Splitting Rates after Single-Embryo Transfers in in Vitro Fertilization N. Engl. J. Med., June 5, 2003; 348(23): 2366 - 2367. [Full Text] [PDF]
|
|
|
|
|
|
S. Abdelmassih, J. Cardoso, V. Abdelmassih, J.A. Dias, R. Abdelmassih, and Z.P. Nagy Laser-assisted ICSI: a novel approach to obtain higher oocyte survival and embryo quality rates Hum. Reprod., October 1, 2002; 17(10): 2694 - 2699. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Fenwick, P. Platteau, A.P. Murdoch, and M. Herbert Time from insemination to first cleavage predicts developmental competence of human preimplantation embryos in vitro Hum. Reprod., February 1, 2002; 17(2): 407 - 412. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. J.R. Menezo and D. Sakkas Monozygotic twinning: is it related to apoptosis in the embryo? Hum. Reprod., January 1, 2002; 17(1): 247 - 248. [Full Text] [PDF]
|
|
|
|
|
|
F. G. Oliveira, V. Abdelmassih, A. L. E. Costa, J. P. Balmaceda, S. Abdelmassih, and R. Abdelmassih Rare association of ovarian implantation site for patients with heterotopic and with primary ectopic pregnancies after ICSI and blastocyst transfer: Case report Hum. Reprod., October 1, 2001; 16(10): 2227 - 2229. [Abstract] [Full Text] [PDF]
|
|