Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Van Blerkom, J.
Right arrow Articles by Davis, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van Blerkom, J.
Right arrow Articles by Davis, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 16, No. 4, 757-764, April 2001
© 2001 European Society of Human Reproduction and Embryology

Differential effects of repeated ovarian stimulation on cytoplasmic and spindle organization in metaphase II mouse oocytes matured in vivo and in vitro

Jonathan Van Blerkom1,2,3 and Patrick Davis1,2

1 Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, CO 80309 and 2 Colorado Reproductive Endocrinology, Rose Medical Center, Denver, CO 80220, USA

The effects of four rounds of ovarian stimulation spaced 1–6 weeks apart on the normality of metaphase II (MII) spindle formation, chromosomal alignment and cytoplasmic organization were examined in intact ovulated mouse oocytes and at MII for oocytes obtained at the germinal vesicle stage from the same ovaries and matured in vitro. The terminal deoxynucleotidyl transferase-mediated dUDP nick-end labelling assay was used to identify DNA strand breaks in chromosomes, and histological studies of ovaries between and at each round of ovarian stimulation were performed. The results demonstrate a progressive and significant increase in the frequency of spindle defects with each round of ovarian stimulation, including those spaced weeks apart. Oocytes with spindle defects were also characterized by the occurrence of detached chromosomes and cytoplasmic asters. In contrast, in-vitro matured oocytes derived from the same ovaries were normal. No evidence of DNA strand breaks with repeated rounds of ovarian stimulation was detected in ovulated or in-vitro matured oocytes. The development and persistence of nodules of hypertrophied granulosa in regions where follicular growth occurs suggest that a progressively increasing proportion of oocytes in the ovulatory pathway may experience an intrafollicular milieu that has negative consequences for competence. The results are discussed with respect to ovarian and oocyte biological ageing and possible adverse implications for human oocyte competence with repeated hyperstimulation.

Key words: meiotic chromosomes/meiotic spindle/oocyte competence/ovarian stimulation

3 To whom correspondence should be addressed at: Department of Molecular, Cellular and Developmental Biology University of Colorado, Boulder, CO 80309, USA. E-mail: vanblerk{at}spot.colorado.edu


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
M.F.G. Verberg, M.J.C. Eijkemans, N.S. Macklon, E.M.E.W. Heijnen, E.B. Baart, F.P. Hohmann, B.C.J.M. Fauser, and F.J. Broekmans
The clinical significance of the retrieval of a low number of oocytes following mild ovarian stimulation for IVF: a meta-analysis
Hum. Reprod. Update, January 1, 2009; 15(1): 5 - 12.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
M.F.G. Verberg, N.S. Macklon, G. Nargund, R. Frydman, P. Devroey, F.J. Broekmans, and B.C.J.M. Fauser
Mild ovarian stimulation for IVF
Hum. Reprod. Update, January 1, 2009; 15(1): 13 - 29.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Z. Han, Y. G. Chung, S. Gao, and K. E. Latham
Maternal Factors Controlling Blastomere Fragmentation in Early Mouse Embryos
Biol Reprod, March 1, 2005; 72(3): 612 - 618.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. Roberts, A. Iatropoulou, D. Ciantar, J. Stark, D. L. Becker, S. Franks, and K. Hardy
Follicle-Stimulating Hormone Affects Metaphase I Chromosome Alignment and Increases Aneuploidy in Mouse Oocytes Matured in Vitro
Biol Reprod, January 1, 2005; 72(1): 107 - 118.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Ziebe, S. Bangsboll, K.L.T. Schmidt, A. Loft, A. Lindhard, and A. Nyboe Andersen
Embryo quality in natural versus stimulated IVF cycles
Hum. Reprod., June 1, 2004; 19(6): 1457 - 1460.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
F. Miyara, F.-X. Aubriot, A. Glissant, C. Nathan, S. Douard, A. Stanovici, F. Herve, M. Dumont-Hassan, A. L. Meur, P. Cohen-Bacrie, et al.
Multiparameter analysis of human oocytes at metaphase II stage after IVF failure in non-male infertility
Hum. Reprod., July 1, 2003; 18(7): 1494 - 1503.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. M.H. Combelles and D. F. Albertini
Assessment of Oocyte Quality Following Repeated Gonadotropin Stimulation in the Mouse
Biol Reprod, March 1, 2003; 68(3): 812 - 821.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.