Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Amer, M.
Right arrow Articles by Zohdy, W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Amer, M.
Right arrow Articles by Zohdy, W.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 16, No. 7, 1427-1432, July 2001
© 2001 European Society of Human Reproduction and Embryology

May–Grünwald–Giemsa stain for detection of spermatogenic cells in the ejaculate: a simple predictive parameter for successful testicular sperm retrieval

Medhat Amer1,2,4, Taha Abd Elnasser1,2, Shawky El Haggar1, Taymour Mostafa1, Geirgis Abdel-Malak2,3 and Wael Zohdy1,2

1 Andrology Department, Cairo University Hospital, Cairo, 2 Adam International Clinic, Guiza and 3 AI/ET Department, Animal Reproduction Research Institute, Guiza, Egypt

BACKGROUND: Testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI) have become standard treatments for patients with non-obstructive azoospermia. A diagnostic testicular biopsy for histopathological examination is not always predictive of TESE outcome. Moreover, it is not without potential complications. The aim of this study was to determine the value of various clinical and laboratory parameters, particularly identification of seminal spermatids using May–Grünwald–Giemsa (MGG) stain in predicting TESE results. METHODS: A total of 100 patients with non-obstructive azoospermia was subjected to clinical examination, serum FSH measurement, identification of seminal spermatids and spermatocytes using MGG staining and TESE with multiple testicular sampling. Spermatozoa were retrieved from 49% of patients. Results of TESE were compared with previous parameters in addition to histopathology. RESULTS: Testicular histopathology was, in general, an inaccurate parameter, and identification of testicular spermatids by histology predicted successful TESE in only 74% of cases. Testicular volume and serum FSH concentration also had poor predictive values. Round spermatids were identified in the ejaculate of 83.7% of TESE-positive cases, and in 22% of TESE-negative cases. CONCLUSIONS: The detection of round spermatids in semen by MGG staining provides the greatest predictive value for successful testicular sperm retrieval, and also has the advantages of simplicity, low cost and availability.

Key words: azoospermia/intracytoplasmic sperm injection/May-Grünwald-Giemsa stain/spermatids/sperm extraction

4 To whom correspondence should be addressed at: Adam International Clinic, 20 Aden street, P. O. Box 12411, Mohandessin, Guiza, Egypt. E-mail: mkamer{at}mednet3.camed.eun.eg


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
I. Koscinski, C. Wittemer, J.M. Rigot, M. De Almeida, E. Hermant, and A. Defossez
Seminal haploid cell detection by flow cytometry in non-obstructive azoospermia: a good predictive parameter for testicular sperm extraction
Hum. Reprod., July 1, 2005; 20(7): 1915 - 1920.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Sousa, N. Cremades, J. Silva, C. Oliveira, L. Ferraz, J.T. da Silva, P. Viana, and A. Barros
Predictive value of testicular histology in secretory azoospermic subgroups and clinical outcome after microinjection of fresh and frozen-thawed sperm and spermatids
Hum. Reprod., July 1, 2002; 17(7): 1800 - 1810.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.