Human Reproduction, Vol. 16, No. 7, 1464-1468,
July 2001
© 2001 European Society of Human Reproduction and Embryology
Developmental ability of human oocytes with or without birefringent spindles imaged by Polscope before insemination
1 Division of Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, Women & Infants Hospital of Rhode Island, Brown University School of Medicine, Providence, RI, 2 Division of Reproductive Endocrinology, Department of Obstetrics and Gynecology, New England Medical Center, Boston, MA and 3 Marine Biological Laboratory, Woods Hole, MA, USA
BACKGROUND: Birefringent spindles imaged with the Polscope can predict fertilization rates after intracytoplasmic sperm injection (ICSI). The present study examined the development of human oocytes with or without birefringent spindles, imaged with the Polscope before ICSI. METHODS: Oocytes were obtained from stimulated ovaries of consenting patients undergoing oocyte retrieval for ICSI. Spindles were imaged with the Polscope combined with a computerized image analysis system. After imaging and ICSI, oocytes with or without spindles were cultured separately for examination of fertilization and embryo development. A total of 1544 oocytes from 136 cycles were examined with the Polscope and inseminated by ICSI. RESULTS: Spindles were imaged in 82% of oocytes. After ICSI, more oocytes (P < 0.05) with spindles (69.4%) fertilized normally, forming 2 pronuclei, than oocytes without spindles (62.9%). At day 3, more oocytes (P < 0.01) with spindles (66.3%) developed to 4–11 cell stages than oocytes without spindles (55.4%). Significantly more (P < 0.001) oocytes with spindles developed to morula and blastocyst by day 5 (51.1 versus 30.3%) and day 6 (53.2 versus 29.3%) compared with oocytes without spindles. CONCLUSIONS: The results indicate that the presence of a birefringent spindle in human oocytes can predict not only higher fertilization rate, but also higher embryo developmental competence.
Key words: embryo development/human oocytes/Polscope/spindles
4 To whom correspondence should be addressed at: IVF Laboratory, Department of Obstetrics and Gynecology, Women & Infants Hospital of Rhode Island, 1 Blackstone Place, 1st Floor, Providence RI 02903, USA. E-mail: wangweihua12{at}hotmail.com
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Dupont, B. D. Bavister, D. R. Armant, and C. A. Brenner Rhesus macaque embryos derived from MI oocytes maturing after retrieval display high rates of chromosomal anomalies Hum. Reprod., April 1, 2009; 24(4): 929 - 935. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-S. Hyun, J.-H. Cha, W.-Y. Son, S.-H. Yoon, K.-A. Kim, and J.-H. Lim Optimal ICSI timing after the first polar body extrusion in in vitro matured human oocytes Hum. Reprod., July 1, 2007; 22(7): 1991 - 1995. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-t. Zeng, Z. Ren, W. S.B. Yeung, Y.-m. Shu, Y.-w. Xu, G.-l. Zhuang, and X.-y. Liang Low mitochondrial DNA and ATP contents contribute to the absence of birefringent spindle imaged with PolScope in in vitro matured human oocytes Hum. Reprod., June 1, 2007; 22(6): 1681 - 1686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Moon, B.-C. Jee, S.-Y. Ku, C.-S. Suh, S.-H. Kim, Y.-M. Choi, J.-G. Kim, and S.-Y. Moon Spindle positions and their distributions in in vivo and in vitro matured mouse oocytes Hum. Reprod., August 1, 2005; 20(8): 2207 - 2210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Shen, T. Stalf, C. Mehnert, U. Eichenlaub-Ritter, and H.-R. Tinneberg High magnitude of light retardation by the zona pellucida is associated with conception cycles Hum. Reprod., June 1, 2005; 20(6): 1596 - 1606. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Bianchi, G. Coticchio, L. Fava, C. Flamigni, and A. Borini Meiotic spindle imaging in human oocytes frozen with a slow freezing procedure involving high sucrose concentration Hum. Reprod., April 1, 2005; 20(4): 1078 - 1083. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Cohen, M. Malcov, T. Schwartz, N. Mey-Raz, A. Carmon, T. Cohen, J.B. Lessing, A. Amit, and F. Azem Spindle imaging: a new marker for optimal timing of ICSI? Hum. Reprod., March 1, 2004; 19(3): 649 - 654. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Rienzi, F. Martinez, F. Ubaldi, M.G. Minasi, M. Iacobelli, J. Tesarik, and E. Greco Polscope analysis of meiotic spindle changes in living metaphase II human oocytes during the freezing and thawing procedures Hum. Reprod., March 1, 2004; 19(3): 655 - 659. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cooke, J.P.P. Tyler, and G.L. Driscoll Meiotic spindle location and identification and its effect on embryonic cleavage plane and early development Hum. Reprod., November 1, 2003; 18(11): 2397 - 2405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Rienzi, F. Ubaldi, F. Martinez, M. Iacobelli, M.G. Minasi, S. Ferrero, J. Tesarik, and E. Greco Relationship between meiotic spindle location with regard to the polar body position and oocyte developmental potential after ICSI Hum. Reprod., June 1, 2003; 18(6): 1289 - 1293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Moon, C.-S. Hyun, S.-W. Lee, W.-Y. Son, S.-H. Yoon, and J.-H. Lim Visualization of the metaphase II meiotic spindle in living human oocytes using the Polscope enables the prediction of embryonic developmental competence after ICSI Hum. Reprod., April 1, 2003; 18(4): 817 - 820. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W.-H. Wang, L. Meng, R. J. Hackett, R. Odenbourg, and D. L. Keefe Limited recovery of meiotic spindles in living human oocytes after cooling-rewarming observed using polarized light microscopy Hum. Reprod., November 1, 2001; 16(11): 2374 - 2378. [Abstract] [Full Text] [PDF]
|
|