Human Reproduction, Vol. 16, No. 8, 1736-1743,
August 2001
© 2001 European Society of Human Reproduction and Embryology
Peritoneal endometriosis: validation of an in-vivo model
1 Institute of Anatomy and 3 Department of Gynaecology, University Hospital Essen, 45122 Essen and 2 Female Health Care Research, Schering AG, 13342 Berlin, Germany
BACKGROUND: The current medical treatment of endometriosis, a common gynaecological disease, is still associated with a high recurrence rate. To establish an appropriate in-vivo model to evaluate new therapeutic strategies we validated the nude mouse model for the intraperitoneal cultivation of human endometrial tissue. METHODS: Human endometrium of the proliferative phase was implanted into the peritoneal cavity of normal cycling and ovariectomized athymic mice and of cycling non-obese diabetic (NOD)-severe combined immuno-deficiency (SCID) mice. Morphology, proliferation, differentiation, and angiogenesis in the ectopic endometrium at different time points after implantation was investigated. RESULTS: Adhesion of endometrial fragments was observed from day 2 onwards. The lesions persisted for up to 28 days revealing a well preserved glandular morphology. The glandular epithelium maintained cytokeratin expression even after 14 days of culture. With progressing culture, glands exhibited vimentin staining in combination with a decrease of surrounding stromal cells. Proliferation of glandular epithelium could be demonstrated throughout the investigated period of 28 days, whereas expression of oestrogen and progesterone receptors was maintained only in endometriotic lesions grown in cycling but not in ovariectomized mice. Neoangiogenesis occurred from day 4 onwards, independent from the intraperitoneal localization of the ectopic lesions. CONCLUSIONS: This in-vivo model is a promising tool to test the effect of compounds such as different hormone agonists/antagonists or anti-angiogenic factors to develop new therapeutic concepts in endometriosis.
Key words: angiogenesis/ectopic endometrium/endometriosis/NOD-SCID mouse/nude mouse
4 To whom correspondence should be addressed. E-mail: ruth.gruemmer{at}uni-essen.de
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. L. Bruner-Tran, K. G. Osteen, and A. J. Duleba Simvastatin Protects against the Development of Endometriosis in a Nude Mouse Model J. Clin. Endocrinol. Metab., July 1, 2009; 94(7): 2489 - 2494. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-L. Alvarez Gonzalez, F. Frankenne, C. Galant, E. Marbaix, J.-M. Foidart, M. Nisolle, and A. Beliard Mixed origin of neovascularization of human endometrial grafts in immunodeficient mouse models Hum. Reprod., June 9, 2009; (2009) dep203v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Van Langendonckt, J. Donnez, S. Defrere, G. A.J. Dunselman, and P. G. Groothuis Antiangiogenic and vascular-disrupting agents in endometriosis: pitfalls and promises Mol. Hum. Reprod., May 1, 2008; 14(5): 259 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. Becker, N. Rohwer, T. Funakoshi, T. Cramer, W. Bernhardt, A. Birsner, J. Folkman, and R. J. D'Amato 2-Methoxyestradiol Inhibits Hypoxia-Inducible Factor-1{alpha} and Suppresses Growth of Lesions in a Mouse Model of Endometriosis Am. J. Pathol., February 1, 2008; 172(2): 534 - 544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Sha, D. Wu, L. Zhang, X. Chen, M. Lei, H. Sun, S. Lin, and J. Lang Differentially expressed genes in human endometrial endothelial cells derived from eutopic endometrium of patients with endometriosis compared with those from patients without endometriosis Hum. Reprod., December 1, 2007; 22(12): 3159 - 3169. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. W. Laschke and M. D. Menger In vitro and in vivo approaches to study angiogenesis in the pathophysiology and therapy of endometriosis Hum. Reprod. Update, July 1, 2007; 13(4): 331 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Masuda, T. Maruyama, E. Hiratsu, J. Yamane, A. Iwanami, T. Nagashima, M. Ono, H. Miyoshi, H. J. Okano, M. Ito, et al. Noninvasive and real-time assessment of reconstructed functional human endometrium in NOD/SCID/{gamma}Formula immunodeficient mice PNAS, February 6, 2007; 104(6): 1925 - 1930. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. A. Harris Estrogen Receptor-{beta}: Recent Lessons from in Vivo Studies Mol. Endocrinol., January 1, 2007; 21(1): 1 - 13. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Grummer Animal models in endometriosis research Hum. Reprod. Update, September 1, 2006; 12(5): 641 - 649. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Becker, R. D. Wright, R. Satchi-Fainaro, T. Funakoshi, J. Folkman, A. L. Kung, and R. J. D'Amato A Novel Noninvasive Model of Endometriosis for Monitoring the Efficacy of Antiangiogenic Therapy Am. J. Pathol., June 1, 2006; 168(6): 2074 - 2084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Defrere, A. Van Langendonckt, R. G. Ramos, M. Jouret, M. Mettlen, and J. Donnez Quantification of endometriotic lesions in a murine model by fluorimetric and morphometric analyses Hum. Reprod., March 1, 2006; 21(3): 810 - 817. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-J. Lin, M.-D. Lai, H.-Y. Lei, and L.-Y. C. Wing Neutrophils and Macrophages Promote Angiogenesis in the Early Stage of Endometriosis in a Mouse Model Endocrinology, March 1, 2006; 147(3): 1278 - 1286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Matsuura-Sawada, T. Murakami, Y. Ozawa, H. Nabeshima, J.-i. Akahira, Y. Sato, Y. Koyanagi, M. Ito, Y. Terada, and K. Okamura Reproduction of menstrual changes in transplanted human endometrial tissue in immunodeficient mice Hum. Reprod., June 1, 2005; 20(6): 1477 - 1484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S Hombach-Klonisch, A Kehlen, P A Fowler, B Huppertz, J F Jugert, G Bischoff, E Schluter, J Buchmann, and T Klonisch Regulation of functional steroid receptors and ligand-induced responses in telomerase-immortalized human endometrial epithelial cells J. Mol. Endocrinol., April 1, 2005; 34(2): 517 - 534. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. A. Harris, K. L. Bruner-Tran, X. Zhang, K. G. Osteen, and C. R. Lyttle A selective estrogen receptor-{beta} agonist causes lesion regression in an experimentally induced model of endometriosis Hum. Reprod., April 1, 2005; 20(4): 936 - 941. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Absenger, H. Hess-Stumpp, B. Kreft, J. Kratzschmar, B. Haendler, N. Schutze, P.-A. Regidor, and E. Winterhager Cyr61, a deregulated gene in endometriosis Mol. Hum. Reprod., June 1, 2004; 10(6): 399 - 407. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Nap, A. W. Griffioen, G. A. J. Dunselman, J. C. A. Bouma-Ter Steege, V. L. J. L. Thijssen, J. L. H. Evers, and P. G. Groothuis Antiangiogenesis Therapy for Endometriosis J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1089 - 1095. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. L. Hull, D. S. Charnock-Jones, C. L. K. Chan, K. L. Bruner-Tran, K. G. Osteen, B. D. M. Tom, T.-P. D. Fan, and S. K. Smith Antiangiogenic Agents Are Effective Inhibitors of Endometriosis J. Clin. Endocrinol. Metab., June 1, 2003; 88(6): 2889 - 2899. [Abstract] [Full Text] [PDF]
|
|