Human Reproduction, Vol. 16, No. 9, 1802-1808,
September 2001
© 2001 European Society of Human Reproduction and Embryology
Apoptosis in endometrial glandular and stromal cells in women with and without endometriosis
1 Institute for the Study and Treatment of Endometriosis, Oak Brook, IL 60523, 2 Rush Medical College, Chicago, IL 60612 and 3 Department of Pathology, Elmhurst Hospital, Elmhurst, IL, 60126, USA
BACKGROUND: The aetiology of endometriosis is unknown. Ectopic dissemination of the endometrial cells gives origin to endometriotic lesions, but occurs in women with and without endometriosis. It has been suggested that increased ectopic cell survival facilitates their implantation. The objectives of this study were to evaluate endometrial apoptosis in women with endometriosis according to: (i) cyclic changes, (ii) glandular and stromal contribution, and (iii) stage of the disease. METHODS: The subjects were women undergoing diagnostic laparoscopy and endometrial biopsies for suspected endometriosis. Spontaneous apoptosis was evaluated using TdT-mediated dUTP-biotin nick end-labelling (TUNEL) assay. Apoptotic cells per 10 mm2 (apoptotic index) in an area of 10–50 mm2 in 5 µm endometrial tissue sections were counted and location of these cells was recorded. RESULTS: The apoptotic index in glandular epithelium was lower in endometriosis than controls (26.0 ± 5.5 versus 51.2 ± 9.7, P = 0.03) but not in the stroma (36.3 ± 6.4 versus 48.4 ± 11.3, NS). In controls, apoptosis was highest during the late secretory/menstrual and early proliferative phases and cyclic variability was apparent. In endometriosis, this cyclic variability was lost. There was a trend toward decreased apoptosis with increasing stage of the disease, but the differences lacked statistical significance. CONCLUSIONS: Spontaneous apoptosis is decreased in the endometrial glands in women with endometriosis, especially during late secretory/menstrual and early proliferative phases of the cycle. This may indicate increased viability of endometrial cells shed during menses, facilitating their ectopic survival and implantation.
Key words: apoptosis/endometrial glands/endometriosis/stroma
4 To whom correspondence should be addressed at: Rush Medical College, Chicago, IL 60612.E-mail: wpdmowski{at}oakbrookfertility.com
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Z. Zhao, P. M. Pollock, S. Thomas, S. A. Treloar, D. R. Nyholt, and G. W. Montgomery Common variation in the fibroblast growth factor receptor 2 gene is not associated with endometriosis risk Hum. Reprod., July 1, 2008; 23(7): 1661 - 1668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-H. Yang, M.-Y. Wu, C.-D. Chen, M.-J. Chen, Y.-S. Yang, and H.-N. Ho Altered apoptosis and proliferation in endometrial stromal cells of women with adenomyosis Hum. Reprod., April 1, 2007; 22(4): 945 - 952. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Xu, J. Ding, G. Rao, J. Shen, R. A. Prinz, N. Rana, and W.P. Dmowski Estradiol induces heparanase-1 expression and heparan sulphate proteoglycan degradation in human endometrium Hum. Reprod., April 1, 2007; 22(4): 927 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ulukus, H. Cakmak, and A. Arici The Role of Endometrium in Endometriosis Reproductive Sciences, October 1, 2006; 13(7): 467 - 476. [Abstract] [PDF]
|
|
|
|
 |
|
 J J Brosens and B Gellersen Death or survival - progesterone-dependent cell fate decisions in the human endometrial stroma. J. Mol. Endocrinol., June 1, 2006; 36(3): 389 - 398. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Zhang and B. C. Paria Importance of Uterine Cell Death, Renewal, and Their Hormonal Regulation in Hamsters that Show Progesterone-Dependent Implantation Endocrinology, May 1, 2006; 147(5): 2215 - 2227. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Izawa, T. Harada, I. Deura, F. Taniguchi, T. Iwabe, and N. Terakawa Drug-induced apoptosis was markedly attenuated in endometriotic stromal cells Hum. Reprod., March 1, 2006; 21(3): 600 - 604. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Labied, T. Kajihara, P. A. Madureira, L. Fusi, M. C. Jones, J. M. Higham, R. Varshochi, J. M. Francis, G. Zoumpoulidou, A. Essafi, et al. Progestins Regulate the Expression and Activity of the Forkhead Transcription Factor FOXO1 in Differentiating Human Endometrium Mol. Endocrinol., January 1, 2006; 20(1): 35 - 44. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Rojas-Cartagena, C. B. Appleyard, O. I. Santiago, and I. Flores Experimental Intestinal Endometriosis Is Characterized by Increased Levels of Soluble TNFRSF1B and Downregulation of Tnfrsf1a and Tnfrsf1b Gene Expression Biol Reprod, December 1, 2005; 73(6): 1211 - 1218. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Matsuzaki, M. Canis, J.L. Pouly, P. Dechelotte, K. Okamura, and G. Mage The macrophage stimulating protein/RON system: a potential novel target for prevention and treatment of endometriosis Mol. Hum. Reprod., May 1, 2005; 11(5): 345 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Nishida, K. Nasu, T. Ueda, J. Fukuda, N. Takai, and I. Miyakawa Endometriotic cells are resistant to interferon-{gamma}-induced cell growth inhibition and apoptosis: a possible mechanism involved in the pathogenesis of endometriosis Mol. Hum. Reprod., January 1, 2005; 11(1): 29 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. M. D'HOOGHE, C KYAMA, S DEBROCK, C MEULEMAN, and J. M. MWENDA Future Directions in Endometriosis Research Ann. N.Y. Acad. Sci., December 1, 2004; 1034(1): 316 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. G. Goumenou, I. M. Matalliotakis, M. Tzardi, Y. G. Fragouli, N. G. Mahutte, and A. Arici Apoptosis and Differential Expression of Apoptosis-Related Proteins in Endometriotic Glandular and Stromal Cells Reproductive Sciences, July 1, 2004; 11(5): 318 - 322. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Harada, A. Kaponis, T. Iwabe, F. Taniguchi, G. Makrydimas, N. Sofikitis, M. Paschopoulos, E. Paraskevaidis, and N. Terakawa Apoptosis in human endometrium and endometriosis Hum. Reprod. Update, January 1, 2004; 10(1): 29 - 38. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Leyendecker, M. Herbertz, G. Kunz, and G. Mall Endometriosis results from the dislocation of basal endometrium Hum. Reprod., October 1, 2002; 17(10): 2725 - 2736. [Abstract] [Full Text] [PDF]
|
|