Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Cremades, N.
Right arrow Articles by Barros, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cremades, N.
Right arrow Articles by Barros, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 16, No. 9, 1938-1944, September 2001
© 2001 European Society of Human Reproduction and Embryology

Developmental potential of elongating and elongated spermatids obtained after in-vitro maturation of isolated round spermatids

Nieves Cremades3, Mário Sousa2,3,4,4, Rafael Bernabeu1 and Alberto Barros2,3

1 Instituto Bernabeu de Fertilidad y Ginecologia, Avda. de la Albufereta 31, 03016 Alicante, Spain, 2 Department of Medical Genetics, Faculty of Medicine, Porto, 3 Centre for Reproductive Genetics, Porto and 4 Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar, State University of Porto, Lg. Prof. Abel Salazar 2, 4099-003 Porto, Portugal

BACKGROUND: Round spermatid injections are associated with disappointing clinical outcomes, and although these cells have been shown to mature into late spermatids in vitro, the developmental potential of such gametes remains to be demonstrated. METHODS: Round spermatids were isolated from 12 testicle samples of patients with obstructive azoospermia, hypoplasia, complete maturation arrest, and incomplete Sertoli cell-only syndrome. They were cultured for 7 days at 32°C, 5% CO2in air, in microdrops of Vero cell-conditioned medium containing 10% synthetic serum substitute. RESULTS: From the 238 round spermatids cultured, 25.2% attained the elongating and 5.5% the elongated spermatid stage (3–4 days per step). Relatively higher maturation rates were found in cases with obstructive azoospermia, but differences were significant only for elongated spermatids (9.3%). No differences were found in maturation rates between cases with non-obstructive azoospermia (4.3% of elongated spermatids). Experimental microinjections with elongating and elongated spermatids revealed a low fertilization rate (40.9%) but a normal blastocyst formation rate (60%). CONCLUSIONS: Late spermatids resulting from in-vitro culture of round spermatids in conditioned medium, either in controls in cases with a spermiogenetic block, appeared able to successfully fertilize the human oocyte and elicit normal embryo development.

Key words: in-vitro maturation/non-obstructive azoospermia/round spermatids

4 To whom correspondence should be addressed. E-mail: msousa{at}icbas.up.pt


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
R. Sa, R. Neves, S. Fernandes, C. Alves, F. Carvalho, J. Silva, N. Cremades, I. Malheiro, A. Barros, and M. Sousa
Cytological and Expression Studies and Quantitative Analysis of the Temporal and Stage-Specific Effects of Follicle-Stimulating Hormone and Testosterone During Cocultures of the Normal Human Seminiferous Epithelium
Biol Reprod, November 1, 2008; 79(5): 962 - 975.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
V. Roulet, H. Denis, C. Staub, A. Le Tortorec, B. Delaleu, A.P. Satie, J.J. Patard, B. Jegou, and N. Dejucq-Rainsford
Human testis in organotypic culture: application for basic or clinical research
Hum. Reprod., June 1, 2006; 21(6): 1564 - 1575.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
N. Sofikitis, E. Pappas, A. Kawatani, D. Baltogiannis, D. Loutradis, N. Kanakas, D. Giannakis, F. Dimitriadis, K. Tsoukanelis, I. Georgiou, et al.
Efforts to create an artificial testis: culture systems of male germ cells under biochemical conditions resembling the seminiferous tubular biochemical environment
Hum. Reprod. Update, May 1, 2005; 11(3): 229 - 259.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. J. Pinho, R. Neves, P. Costa, C. Ferras, M. Sousa, C. Alves, C. Almeida, S. Fernandes, J. Silva, L. Ferras, et al.
Unique t(Y;1)(q12;q12) reciprocal translocation with loss of the heterochromatic region of chromosome 1 in a male with azoospermia due to meiotic arrest: a case report
Hum. Reprod., March 1, 2005; 20(3): 689 - 696.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Sousa, N. Cremades, J. Silva, C. Oliveira, L. Ferraz, J.T. da Silva, P. Viana, and A. Barros
Predictive value of testicular histology in secretory azoospermic subgroups and clinical outcome after microinjection of fresh and frozen-thawed sperm and spermatids
Hum. Reprod., July 1, 2002; 17(7): 1800 - 1810.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.