Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (28)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Blake, D.A.
Right arrow Articles by Wikland, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blake, D.A.
Right arrow Articles by Wikland, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 16, No. 9, 1959-1964, September 2001
© 2001 European Society of Human Reproduction and Embryology

Laser zona pellucida thinning—an alternative approach to assisted hatching

D.A. Blake1,3,3, A.S. Forsberg1, B.R. Johansson2 and M. Wikland1

1 Fertilitetscentrum, Carlander's Hospital, Göteborg, 2 Institute of Anatomy and Cell Biology, University of Göteborg, Sweden and 3 Glycoscience Research Centre, Auckland University of Technology, Auckland, New Zealand

BACKGROUND: The purpose of this study was to assess the efficacy and hatching characteristics of in-vitro cultured human embryos subjected to laser zona pellucida thinning. METHOD: Zona thinning was performed on 110 embryos using a non-contact 1.48 µm diode laser and the hatch rate in vitro was compared with 42 control embryos. Variation of zona thickness and degree of zona expansion was assessed. Scanning electron microscopy was performed on embryos entrapped during hatching to identify the site of hatching. RESULTS: The rate of hatching was significantly higher in laser thinned blastocysts compared with control embryos (68 versus 33% respectively, P < 0.01). Laser thinning increased the variation of zona thickness in embryos from 11.6–27.3%. Natural zona thinning occurred in 92% of laser thinned hatching blastocysts and 100% of control embryos. CONCLUSION: These results suggest that laser zona thinning is effective and may provide significant advantages over conventional assisted hatching techniques, which create holes.

Key words: assisted hatching/in-vitro fertilization/laser/micromanipulation/zona pellucida

3 To whom correspondence should be addressed at: Glycoscience Research Centre, Auckland University of Technology, Private Bag 92006, Auckland 1020, New Zealand. Email: debbie.blake{at}aut.ac.nz


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
C. Sifer, A. Sellami, C. Poncelet, P. Kulski, B. Martin-Pont, J. Bottero, R. Porcher, I. Cedrin-Durnerin, J.N. Hugues, and J.P. Wolf
A prospective randomized study to assess the benefit of partial zona pellucida digestion before frozen-thawed embryo transfers
Hum. Reprod., September 1, 2006; 21(9): 2384 - 2389.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
N. Frydman, S. Madoux, L. Hesters, C. Duvernoy, E. Feyereisen, A. Le Du, G. Tachdjian, R. Frydman, and R. Fanchin
A randomized double-blind controlled study on the efficacy of laser zona pellucida thinning on live birth rates in cases of advanced female age
Hum. Reprod., August 1, 2006; 21(8): 2131 - 2135.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
T. Ebner, M. Moser, and G. Tews
Possible applications of a non-contact 1.48 {micro}m wavelength diode laser in assisted reproduction technologies
Hum. Reprod. Update, July 1, 2005; 11(4): 425 - 435.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
Q.F. Lyu, L.Q. Wu, Y.P. Li, Q. Pan, D.E. Liu, K. Xia, D.S. Liang, F. Cai, Z.G. Long, H.P. Dai, et al.
An improved mechanical technique for assisted hatching
Hum. Reprod., June 1, 2005; 20(6): 1619 - 1623.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
E. H. Y. Ng, F. Naveed, E. Y. L. Lau, W. S. B. Yeung, C. C. W. Chan, O. S. Tang, and P. C. Ho
A randomized double-blind controlled study of the efficacy of laser-assisted hatching on implantation and pregnancy rates of frozen-thawed embryo transfer at the cleavage stage
Hum. Reprod., April 1, 2005; 20(4): 979 - 985.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Moser, T. Ebner, M. Sommergruber, U. Gaisswinkler, K. Jesacher, M. Puchner, R. Wiesinger, and G. Tews
Laser-assisted zona pellucida thinning prior to routine ICSI
Hum. Reprod., March 1, 2004; 19(3): 573 - 578.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Abdelmassih, J. Cardoso, V. Abdelmassih, J.A. Dias, R. Abdelmassih, and Z.P. Nagy
Laser-assisted ICSI: a novel approach to obtain higher oocyte survival and embryo quality rates
Hum. Reprod., October 1, 2002; 17(10): 2694 - 2699.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.