Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Dinara, S.
Right arrow Articles by Ishikawa, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dinara, S.
Right arrow Articles by Ishikawa, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 16, No. 9, 1976-1981, September 2001
© 2001 European Society of Human Reproduction and Embryology

Effects of supplementation with free radical scavengers on the survival and fertilization rates of mouse cryopreserved oocytes

S. Dinara, K. Sengoku1,, K. Tamate, M. Horikawa and M. Ishikawa

Department of Obstetrics and Gynecology, Midorigaoka Higashi 2-1-1-1, Asahikawa 078-8510, Japan

BACKGROUND: This study was conducted to investigate the effects of supplementation with free radical scavengers on the survival and fertilization rates of freeze–thawed mouse oocytes. METHODS: Superovulated oocytes with cumulus cells were cryopreserved by slow freezing in propanediol combined with a rapid thawing protocol. The cryopreservation medium was supplemented with the antioxidant enzymes superoxide dismutase (SOD) and catalase, and with the nitric oxide (NO) scavenger, haemoglobin (Hb). RESULTS: The addition of 50 IU/ml SOD showed significantly higher survival and fertilization capabilities compared with control (P < 0.01). Oocyte survival was greatly increased by concomitant addition of SOD with 10 IU/ml catalase (P < 0.01). On the other hand, the NO donor (sodium nitroprusside) inhibited survival and fertilization rates (P < 0.05). Significantly decreased survival and fertilization rates were also observed following the addition of high concentrations (10–3 to 10–6 nmol/l) of the NO synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME). In contrast, significantly better oocyte survival and fertilization rates were detected with low concentrations (10–7 nmol/l) of L-NAME. Oocyte survival potential was significantly increased by addition of Hb (1 µg/ml, P < 0.05). Moreover, oocyte survival and fertilization rates were significantly promoted by the concomitant addition of SOD with Hb (P < 0.01). CONCLUSIONS: These results suggest that supplementation of free radical scavengers, particularly combinations of SOD with NO scavengers in freezing and thawing media, improved the post-thaw survival and fertilization rates of cryopreserved mouse oocytes.

Key words: cryopreservation/catalase/mouse oocytes/nitric oxide/superoxide dismutase

1 To whom correspondence should be addressed. E-mail: ksen{at}asahikawa-med.ac.jp


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
R. Fukuhara, S. Fujii, R. Nakamura, E. Yuzawa, H. Kimura, A. Fukui, and H. Mizunuma
Erythrocytes counteract the negative effects of female ageing on mouse preimplantation embryo development and blastocyst formation
Hum. Reprod., September 1, 2008; 23(9): 2080 - 2085.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
L. M. Mitchell, C.R. Kennedy, and G. M. Hartshorne
Expression of nitric oxide synthase and effect of substrate manipulation of the nitric oxide pathway in mouse ovarian follicles
Hum. Reprod., January 1, 2004; 19(1): 30 - 40.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.