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Human Reproduction, Vol. 17, No. 1, 173-177, January 2002
© 2002 European Society of Human Reproduction and Embryology

Time to pregnancy in relation to semen quality assessed by CASA before and after sperm separation

A.G. Andersen1,2,4, S. Ziebe1, N. Jørgensen2, J.H. Petersen2,3, N.E. Skakkebæk2 and A. Nyboe Andersen1

1 The Fertility Clinic, 2 Department of Growth and Reproduction, Copenhagen University Hospital, Blegdamsvej 9, DK-2100 Copenhagen Ø and 3 Department of Biostatistics, University of Copenhagen, Blegdamsvej 3, DK-2100 Copenhagen N, Denmark

BACKGROUND: In order to provide a reference for infertile men, we defined normal values of semen quality in a population of fertile men, using computer-assisted semen analysis (CASA) before and after sperm separation. Additionally, we investigated the relationship between semen quality and time to pregnancy (TTP). METHODS AND RESULTS: Semen samples were obtained from 315 proven fertile men. The median sperm concentration in fresh samples was 107x106/ml (5–95 percentiles: 16–322x106/ml), the median percentage of motile sperm cells was 65% (14–87%) and the median percentage of progressively motile cells was 37% (5–64%). After density gradient sperm separation, the median total sperm count was 46x106 (4–350x106), the median percentage of motile sperm cells was 77% (16–95%) and the median percentage of progressively motile cells was 63% (11–84%). No significant associations were found between TTP and sperm counts or sperm motility, either before or after sperm separation. This may be due in part to the fact that the study comprised couples with proven fertility. CONCLUSION: We have defined semen parameters, including the results of density gradient separation, in a population of normal fertile men which may be of interest in the evaluation of semen samples from infertile men.

Key words: computer-assisted semen analysis/density gradients/fertile men/time to pregnancy

4 To whom correspondence should be addressed at: Department of Growth and Reproduction, Copenhagen University Hospital, Section GR-5064, Blegdamsvej 9, DK-2100 Copenhagen Ø, Denmark. E-mail: agma{at}get2net.dk


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