Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Macklon, N.S.
Right arrow Articles by Fauser, B.C.J.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Macklon, N.S.
Right arrow Articles by Fauser, B.C.J.M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 17, No. 10, 2700-2705, October 2002
© 2002 European Society of Human Reproduction and Embryology

A prospective randomized comparison of sequential versus monoculture systems for in-vitro human blastocyst development

N.S. Macklon1,3, M.H.E.C. Pieters1, M.A. Hassan1, P.H.M. Jeucken1, M.J.C. Eijkemans2 and B.C.J.M. Fauser1

1 Division of Reproductive Medicine, Department of Obstetrics and Gynaecology and 2 Department of Public Health, Centre for Clinical Decision Sciences, Erasmus Medical Centre, Rotterdam, The Netherlands

BACKGROUND: Extending the period of in-vitro culture to the blastocyst stage may improve implantation rates in IVF treatment. Recognition of the dynamic nature of early embryo metabolism has led to the development of commercially available sequential culture systems. However, their improved efficacy over monoculture systems remains to be demonstrated in prospective studies. METHODS: Embryos obtained from 158 women undergoing IVF treatment were randomized by sealed envelopes to culture in one of three systems: (A) culture for 5 days in our own monoculture medium (Rotterdam medium); (B) culture for 3 days in Rotterdam medium followed by 2 days in fresh Rotterdam medium; (C) culture for 5 days using the commercially available G1/G2 sequential culture system. RESULTS: There were no significant differences in blastulation, implantation or pregnancy rates between the three tested culture systems. CONCLUSION: The employed monoculture system is as effective as the G1/G2 sequential system for the culture of blastocysts for IVF.

Key words: blastocyst/culture medium/IVF/pregnancy rates/randomized controlled trial

3 To whom correspondence should be addressed at: Department of Obstetrics and Gynaecology, Erasmus Medical Center, Dr. Molewaterplein 40, 3015GD Rotterdam, The Netherlands. E-mail: macklon{at}gyna.azr.nl


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
S. Dias, R. McNamee, and A. Vail
Evidence of improving quality of reporting of randomized controlled trials in subfertility
Hum. Reprod., October 1, 2006; 21(10): 2617 - 2627.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. D. Biggers, L. McGinnis, and M. C. Summers
Reply: One-step versus two-step culture of mouse preimplantation embryos
Hum. Reprod., July 1, 2006; 21(7): 1936 - 1939.
[Full Text] [PDF]

Home page
 Hum ReprodHome page
J.D. Biggers, L.K. McGinnis, and J.A. Lawitts
One-step versus two-step culture of mouse preimplantation embryos: is there a difference?
Hum. Reprod., December 1, 2005; 20(12): 3376 - 3384.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.